A1603

Alginate Lyase

powder, ≥10,000 units/g solid

• Synonyme(s) : poly-beta-D-mannuronate lyase
• Numéro CAS: 9024-15-1
• UNSPSC Code: 12352204
• NACRES: NA.54
• Numéro CE : 4.2.2.3 (BRENDA, IUBMB)
• MDL number: MFCD03454593
• Specific activity: ≥10,000 units/g solid

Propriétés

• form: powder
• Quality Segment: 200
• specific activity: ≥10,000 units/g solid
• storage temp.: 2-8°C

Description

General description

Alginate lyases is present in several marine algae, marine mollusks and a vast range of marine and terrestrial bacteria.

Application

Alginate Lyase is used to break down alginate or alginic acid and to reduce viscosity. It is useful during follicle isolation, encapsulation, and culture .

Alginate Lyase has been used:

• in the pre-processing of enzyme
• to de-encapsulate cells
• to digest Pseudomonas exopolysaccharide to facilitate electroporation

Biochem/physiol Actions

Breaks down alginate or alginic acid to smaller molecules and reduces viscosity.

Alginate lyases actively participates in the saccharification of the acidic polysaccharides for the production of bioethanol.

Preparation Note

Add 0.15 mI of enzyme solution (1 un/ml) to 4.5 ml of 0.1% sodium alginate (pH 6.3). Incubate at 37 °C for 30 minutes. Terminate reaction by addition of 4.65 ml of 0.1 N NaOH.

Other Notes

One unit will produce an increase the A_235nm of 1.0 per minute per mL of sodium alginate solution at pH 6.3 at 37 °C

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Informations sur la sécurité

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Classe de stockage: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)