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52412

Sigma-Aldrich

R-Phycoerythrin

BioReagent, passes test for gel electrophoresis

Synonyme(s) :

R-PE, phycobiliprotein

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About This Item

Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Gamme de produits

BioReagent

Niveau de qualité

Concentration

≥10.0 mg/mL (UV)

Technique(s)

electrophoresis: suitable

Fluorescence

λex 542 nm; λem 575 nm in 0.1 M phosphate pH 7.2

Adéquation

passes test for gel electrophoresis

Température de stockage

2-8°C

Description générale

R-Phycoerythrin or R-PE is a fluorescent probe belonging to the group of phycobiliproteins obtained from macroalgae. R-Phycoerythrin (R-PE) is a protein acting as a photosynthetic accessory pigment in red algae (Rhodophyta). It is an oligomeric protein of 240 kDa, with 6 α (about 20 kDa), 6 β (about 20 kDa), and 1 γ (about 30 kDa) subunits. Phycobiliproteins are water-soluble light-harvesting proteins with a high fluorescent property.

Application

R-Phycoerythrin (R-PE) is useful in the laboratory as a fluorescence-based indicator for the presence of cyanobacteria and a variety of immunofluorescence applications. R-PE is a useful fluoroprobe in various electrophoretic procedures. R-Phycoerythrin (R-PE) proteins detect mercury ions in soil and groundwater samples. Phycoerythrin has been widely used in food, cosmetics, immunodiagnostics, and analytical reagents.

Remarque sur l'analyse

The product is suspended in 150 mM sodium phosphate, 60% ammonium sulfate, 1 mM EDTA, 1 mM sodium azide, pH 7.0 and must be dialyzed against conjugation buffer or PBS before conjugation.A566/A280 >4.6, A620/A566 <0.03, A566/A498 <1.5

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves


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Consulter la Bibliothèque de documents

Xiaobin Wang et al.
The Analyst, 144(12), 3892-3897 (2019-05-24)
Mercury, as one of the most prevalent toxic metals released by various natural and anthropogenic processes, causes severe pollution of soil and groundwater. In this work, R-phycoerythrin (R-PE) proteins encapsulated into ZIF-8 composite thin films were prepared via a solid-confinement
R Rossano et al.
Journal of biotechnology, 101(3), 289-293 (2003-03-05)
R-Phycoerythrin (R-PE) is a protein acting as a photosynthetic accessory pigment in red algae (Rodophyta). This protein has gained importance in many biotechnological applications in food science, immunodiagnostic, therapy, cosmetics, protein and cell labelling, and analytical processes. In this paper
Kun Tang et al.
Biochimica et biophysica acta, 1817(7), 1030-1036 (2012-04-03)
Phycobilisomes, the light-harvesting antennas in cyanobacteria and red algae, consist of an allophycocyanin core that is attached to the membrane via a core-membrane linker, and rods comprised of phycocyanin and often also phycoerythrin or phycoerythrocyanin. Phycobiliproteins show excellent energy transfer
R Craig Everroad et al.
Molecular phylogenetics and evolution, 64(3), 381-392 (2012-05-17)
In marine Synechococcus there is evidence for the adaptive evolution of spectrally distinct forms of the major light harvesting pigment phycoerythrin (PE). Recent research has suggested that these spectral forms of PE have a different evolutionary history than the core
Radek Sachl et al.
Biophysical journal, 101(11), L60-L62 (2012-01-21)
Even though superresolution microscopy indicates that size of plasma membrane rafts is <20 nm, those structures have never been observed. Förster resonance energy transfer (FRET) is therefore still the most powerful optical method for characterization of such domains. In this letter

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