Based on the following study, which involved ROS analysis of cells seeded in 6-well plates, it may be possible to use 96-well plates: https://doi.org/10.1016/j.lfs.2018.08.056
21884
5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate
5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate
BioReagent, suitable for fluorescence, ≥85% (HPCE)
Synonyme(s) :
5&6DH-DA, CDFDA, Carboxy-DCFDA
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Formule empirique (notation de Hill):
C25H14Cl2O9
Numéro CAS:
Poids moléculaire :
529.28
Beilstein:
7505106
Numéro MDL:
Code UNSPSC :
12352106
ID de substance PubChem :
Nomenclature NACRES :
NA.32
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Gamme de produits
BioReagent
Niveau de qualité
Essai
≥85% (HPCE)
Pf
210 °C (dec.) (lit.)
Solubilité
DMF: soluble
DMSO: soluble
methanol: soluble
Fluorescence
λex 470 nm; λem 529 nm in 0.1 M Tris pH 8.0 (esterase)
Adéquation
suitable for fluorescence
Température de stockage
−20°C
Chaîne SMILES
CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C4(OC(=O)c5cc(ccc45)C(O)=O)c2cc1Cl.CC(=O)Oc6cc7Oc8cc(OC(C)=O)c(Cl)cc8C9(OC(=O)c%10ccc(cc9%10)C(O)=O)c7cc6Cl
InChI
1S/2C25H14Cl2O9/c1-10(28)33-21-8-19-15(6-17(21)26)25(14-4-3-12(23(30)31)5-13(14)24(32)36-25)16-7-18(27)22(34-11(2)29)9-20(16)35-19;1-10(28)33-21-8-19-15(6-17(21)26)25(14-5-12(23(30)31)3-4-13(14)24(32)36-25)16-7-18(27)22(34-11(2)29)9-20(16)35-19/h2*3-9H,1-2H3,(H,30,31)
Clé InChI
LZMPJBOMBCYYMP-UHFFFAOYSA-N
Description générale
Application
5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate or Carboxy-DCFDA is used as a cell permeate probe to detect intracellular reactive oxygen species (ROS). [3] The anionic fluorescent dye, 5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate is suitable for studying Multi-Drug Resistance (MDR) by target cells.[4]
Conditionnement
Bottomless glass bottle. Contents are inside inserted fused cone.
Remarque sur l'analyse
Excmax: 504 nm (Lit.)
Autres remarques
Intracellular esterase substrate with low sensitivity of fluorescence to intracellar pH[5]
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Organes cibles
Respiratory system
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
dust mask type N95 (US), Eyeshields, Gloves
Faites votre choix parmi les versions les plus récentes :
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
C K Raymond et al.
The Journal of cell biology, 111(3), 877-892 (1990-09-01)
vps3 mutants of the yeast Saccharomyces cerevisiae are impaired in the sorting of newly synthesized soluble vacuolar proteins and in the acidification of the vacuole (Rothman, J. H., and T. H. Stevens. Cell. 47:1041-1051; Rothman, J. H., C. T. Yamashiro
Aileen I Pogue et al.
International journal of molecular sciences, 13(8), 9615-9626 (2012-09-06)
Evolution of reactive oxygen species (ROS), generated during the patho-physiological stress of nervous tissue, has been implicated in the etiology of several progressive human neurological disorders including Alzheimer's disease (AD) and amylotrophic lateral sclerosis (ALS). In this brief communication we
Surgical treatment of the symptomatic juvenile flexible flatfoot condition.
G L Dockery
Clinics in podiatric medicine and surgery, 4(1), 99-117 (1987-01-01)
Tassarut Chaisit et al.
European journal of pharmacology, 795, 50-57 (2016-12-06)
Rhinacanthin-C is a major bioactive naphthoquinone ester found in Rhinacanthus nasutus Kurz (Acanthaceae). This compound has potential therapeutic value as an anticancer and antiviral agent. In this study, we investigated an enhancement effect of rhinacanthin-C on doxorubicin cytotoxicity in human
Guillermo Nicolás Tocchetti et al.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 122, 205-213 (2018-07-10)
Multidrug resistance-associated protein 2 (MRP2) plays a key role in hepatic and intestinal disposition of endo- and xenobiotics. Several therapeutic agents modulate MRP2 activity resulting in pharmacological interactions. Nomegestrol acetate (NMGA) is a progestogen increasingly used in contraceptive formulations. The
-
O produto 21884-100MG pode ser utilizado para medir ROS em leitores de microplacas de 96 poços?
1 answer-
Helpful?
-
Active Filters
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique
