X114

Triton^™ X-114

laboratory grade

• Synonyme(s) : (1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol, Polyethylene glycol tert-octylphenyl ether
• Formule linéaire : (C₂H₄O)n C₁₄H₂₂O, n = 7 or 8
• Numéro CAS: 9036-19-5
• Numéro Beilstein : 2315025
• Numéro MDL: MFCD00128254
• Code UNSPSC : 12161900
• ID de substance PubChem : 24902129
• Nomenclature NACRES : NA.21

Propriétés

• Qualité: laboratory grade
• Niveau de qualité: 200
• Description: non-ionic
• Poids mol.: average mol wt 537
• Composition: t-Oct-C₆H₄-(OCH₂CH₂)_xOH, x = 7-8
• Indice de réfraction: n20/D 1.488 (lit.)
• CMC: 0.2 mM (20-25°C)
• Température de transition: pour point ~−9 °C; cloud point 23 °C
• Densité: 1.058 g/mL at 25 °C (lit.)
• HLB: 12.4
• Chaîne SMILES: CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
• InChI: 1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
• Clé InChI: HNLXNOZHXNSSPN-UHFFFAOYSA-N

Description

Description générale

Triton^™ X-114 is a nonionic detergent. It is a good detergent for solubilizing membranes having reversible phase-separation property useful for the separation of intrinsic membrane proteins. Solution of Triton^™ X-114 is homogeneous at 0°C but phase separation into aqueous phase and a detergent phase occurs above 20°C. Phase separation of proteins in solutions of Triton^™ X-114 has been investigated. This octylphenol ethoxylate is compatible with anionic, cationic, and other nonionic surfactants and chemically stable in most acidic & alkaline solutions.

Application

Triton^™ X-114 has been used:

• To remove endotoxin from solutions of protein using phase separation technique
• In the extraction and purification of DNA from endotoxin
• In the biphasic extraction of lipoproteins from bacterial cell wall
• As a non-ionic detergent with a low cloud point (23 °C) enabling protein solubilization with phase-partitioning of hydrophilic from amphiphilic proteins

Informations légales

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Informations sur la sécurité

• Pictogrammes: GHS05,GHS07,GHS09
• Mention d'avertissement: Danger
• Mentions de danger: H302,H315,H318,H410
• Conseils de prudence: P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): 483.8 °F - closed cup
• Point d'éclair (°C): 251 °C - closed cup
• Équipement de protection individuelle: Eyeshields, Gloves, type ABEK (EN14387) respirator filter