W0625

Wright stain

≤4% purity (HPLC), powder

• Synonyme(s) : Eosin Methylene blue according to Wright
• Numéro CAS: 68988-92-1
• Numéro MDL: MFCD00082143
• Code UNSPSC : 12171500
• Nomenclature NACRES : NA.47

Propriétés

• product name: Wright stain, suitable for blood stain
• Pureté: ≤4% (HPLC)
• Forme: powder
• Concentration: ≤4% (HPLC)
• Couleur: dark green
• Solubilité: methanol: 10 mg/10 mL
• Adéquation: suitable for blood stain
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: room temp
• InChI: 1S/C20H8Br4O5.C16H18N3S/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13/h1-6H,(H2-,25,26,27,28);5-10H,1-4H3/q;+1/p+1
• Clé InChI: AXIKDPDWFVPGOD-UHFFFAOYSA-O

Description

Description générale

Wright′s Stain solution is commercially available and is generally used in hematology. Its preparation involves heating methylene blue with sodium bicarbonate and combining the mixture with eosin. It is alkaline, hence, its concentration is continuously changing due to the progressive oxidizing action of the alkali. Therefore, Giemsa′s stain is added to preserve the azurophilic properties of polychrome dyes.

Application

Wright′s Stain is mainly used for the staining of peripheral blood smears and bone marrow smears.

• It is also used for G-banding and helps in the analysis of poorly spread metaphases.
• It has been used to study the cytogenetics of human hematopoietic cells.
• It has also been employed in the karyotyping of human embryonic stem cells.
• It is also used in the staining of chromosomes.
• It is used for the exudate analysis of white blood cell concentration.

Reported to be superior to May-Grunwald Stain in blood staining.

Caractéristiques et avantages

The purified dyes in Wright′s Stain eliminate inconsistent staining and yield reproducible lot-to-lot chromogenic responses.

Notes préparatoires

Prepared using polychromed (oxidized) methylene blue and eosin Y.

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H302,H319
• Conseils de prudence: P280 - P301 + P312 + P330 - P305 + P351 + P338 - P337 + P313
• Classification des risques: Acute Tox. 4 Oral - Eye Irrit. 2
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves