H3136

Hematoxylin

certified by the Biological Stain Commission

• Synonyme(s) : Natural Black 1
• Formule empirique (notation de Hill): C₁₆H₁₄O₆ · xH₂O
• Numéro CAS: 517-28-2
• Poids moléculaire : 302.28 (anhydrous basis)
• Numéro C.I. (Colour Index): 75290
• Numéro Beilstein : 91400
• Numéro CE : 208-237-3
• Numéro MDL: MFCD00078111
• Code UNSPSC : 12171500
• ID de substance PubChem : 24895554
• Nomenclature NACRES : NA.47

Propriétés

• Qualité: certified by the Biological Stain Commission
• Niveau de qualité: 200
• Forme: powder
• Pf: 200 °C (dec.) (lit.)
• Solubilité: ethanol: 1 mg/mL
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: room temp
• Chaîne SMILES: Oc1cc2C[C@@]3(O)COc4c(O)c(O)ccc4[C@H]3c2cc1O
• InChI: 1S/C16H14O6/c17-10-2-1-8-13-9-4-12(19)11(18)3-7(9)5-16(13,21)6-22-15(8)14(10)20/h1-4,13,17-21H,5-6H2/t13-,16+/m0/s1
• Clé InChI: WZUVPPKBWHMQCE-XJKSGUPXSA-N
• Informations sur le gène: rat ... Adora1(29290) , Adora2a(25369)

Description

Description générale

Hematoxylin is a basophilic dye extracted from the tree Heamatoxylum campechianum. It specifically binds to acidic compounds and thus is useful for staining cell nuclei which contain acidic substances: DNA and RNA. Hematoxylin, when oxidized forms hematein, which has thick pale blue purple colour. Hematoxylin is often used along with eosin, in histological study under light microscope.

Application

Hematoxylin has been used:

• to visualize anatomical features and to detect nucleus locus coeruleus adjacent to the periaqueductal gray region in brainstem samples
• as a nuclear counterstain to visualize the nucleus, adipocytes were counterstained with a hematoxylin reagent
• to stain lung tissue for histological analysis

Adéquation

Certified for use in Weigert′s iron hematoxylin, Mayer′s hemalum, Harris hemalum and in Heidenhain′s technique

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H319
• Conseils de prudence: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Classification des risques: Eye Irrit. 2
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves