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70138

Millipore

Brain Heart Infusion Agar

suitable for microbiology, NutriSelect® Plus, For the cultivation of fastidious, pathogenic bacteria, yeasts, and molds

Synonyme(s) :

BHI Agar

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About This Item

Code UNSPSC :
41171605
Nomenclature NACRES :
NA.74

Niveau de qualité

200

Stérilité

non-sterile

Gamme de produits

BioChemika

Forme

powder

Durée de conservation

limited shelf life, expiry date on the label

Composition

agar, 15 g/L
brain extract, 7.8 g/L
dextrose, 2.0 g/L
disodium phosphate, 2.5 g/L
heart extract, 9.7 g/L
proteose peptone, 10.0 g/L
sodium chloride, 5.0 g/L

Fabricant/nom de marque

NutriSelect® Plus

Technique(s)

microbe id | specific enzyme detection: suitable
microbiological culture: suitable

pH final

7.4±0.2 (25 °C)

pH

7.2-7.6 (25 °C)

Application(s)

clinical testing
environmental
food and beverages
life science and biopharma
water monitoring
microbiology

Température de stockage

10-30°C

Adéquation

Ajellomyces spp.
Blastomyces spp.
Enterococcus spp.
Erysipelothrix spp.
Haemophilus spp.
Pneumococcus spp.
nonselective for Candida spp.
nonselective for Clostridium spp.
nonselective for Escherichia coli
nonselective for Lactobacillus spp.
nonselective for Neisseria spp.
nonselective for Shigella spp.
nonselective for Staphylococcus spp.
nonselective for Streptococcus spp.
nonselective for bacteria (General Media)
nonselective for coliforms

Catégories apparentées

Description générale

Rosenow devised the original medium by adding brain tissue to dextrose agar. These media are nutritious and well buffered to support the growth of a wide range of microorganisms such as Streptococci, Pneumococci, Meningococci, etc. Addition of ascites permits the cultivation of Gonococci. With the addition of 10% defibrinated sheep blood, it is useful for isolation and cultivation of Histoplasma capsulatum and other pathogenic fungi. This medium is to be used for the selective isolation of fastidious fungi (especially of Histoplasma capsulatum and Blastomyces), the growth of the accompanying bacterial and saprophytic yeasts and molds can be almost completely suppressed by adding the following recommended components mixtures: 12 mg Penicillin and 40 ug Streptomycin per liter; 50 mg Chloramphenicol and 500mg Cycloheximide per liter; 50 mg Gentamicin and/or 50 mg Chloramphenicol per liter.
This medium is less suited for identifying haemolytic forms when blood is added due to its glucose content. While handling Histoplasma capsulatum extreme care should be taken to avoid dissemination of its infective spores. The culture should be examined in a closed filtered air cabinet.

Application

BHI agar is a general-purpose medium used for primary isolation of pathogenic aerobic bacteria from clinical specimens and for the cultivation and maintenance of fastidious microorganisms from food, animal feed, water, and other samples
Defibrinated sheep blood added to the basal medium provides essential growth factors for the more fastidious fungal organisms.

Notes préparatoires

Suspend 52 g in 1 litre of distilled water. Boil to dissolve the medium completely. Distribute into tubes or flasks and sterilize by autoclaving at 121°C for 15 minutes.

Autres remarques

Selection for vancomycin resistance in clinical isolates of Staph. haemolyticus

Note de bas de page

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Informations légales

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
BCCM0192
BCCK8190
BCCK0800
BCCH9536
BCCG3725

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