11388908910
Roche
Biotin-16-UTP
pkg of 25 μL (250 nmol; 10mM)
Synonyme(s) :
Biotin-16-UTP, biotin
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About This Item
Code UNSPSC :
41116100
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Essai
97.7% (HPLC)
Niveau de qualité
Forme
solution
Poids mol.
Mr 987.5 (biotin-16-UTP-Li4)
Conditionnement
pkg of 25 μL (250 nmol; 10mM)
Fabricant/nom de marque
Roche
Couleur
colorless
Solubilité
water: miscible
Conditions d'expédition
dry ice
Température de stockage
−20°C
Description générale
For biotin-16-UTP, biotin is bound to uridine triphosphate via an amide linkage. Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace UTP in the in vitro transcription reaction for RNA labeling.[1] Linearized template DNA with T7, SP6, or T3 promoter is in vitro-transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and Biotin-16-UTP, respectively.
Application
Biotin-labeled RNA can be used as a hybridization probe for:
The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by enzyme linked immuno sorbent assay (ELISA) using a Streptavidin-AP conjugate.
The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by enzyme linked immuno sorbent assay (ELISA) using a Streptavidin-AP conjugate.
Qualité
Typical analysis: At least 85% Biotin-16-UTP (HPLC, area%).
Forme physique
Biotin-16-UTP, tetralithium salt, 10mM solution
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
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