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PC408

Sigma-Aldrich

Anti-Green Fluorescent Protein (26-39) Rabbit pAb

liquid, Calbiochem®

Synonyme(s) :

Anti-GFP

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43

Source biologique

rabbit

Niveau de qualité

100

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

liquid

Contient

≤0.1% sodium azide as preservative

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze
avoid repeated freeze/thaw cycles

Isotype

IgG

Conditions d'expédition

wet ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Description générale

Anti-Green Fluorescent Protein (26-39), rabbit polyclonal, recognizes the ~28 kDa GFP and some GFP fusion proteins. It is validated for use in Western blotting and immunocytochemistry.
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~28 kDa GFP and some GFP fusion proteins.

Recognizes the ~28 kDa GFP and some GFP fusion proteins.

Immunogène

Aequora victoria
a synthetic peptide corresponding to amino acids 26-39 of Aequorea victoria wild-type green fluorescent protein

Application

Immunoblotting (1:2000)

Immunocytochemistry (1:200)

Conditionnement

Please refer to vial label for lot-specific concentration.

Avertissement

Toxicity: Standard Handling (A)

Forme physique

In PBS, 1 mg/ml BSA.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Autres remarques

Cubitt, A.B., et al. 1995. Trends Biochem. Sci.20, 448.
This sequence is completely conserved between GFP and the modified and enhanced Blue, Green, and Yellow FP variants. This antibody has been successfully used in immunoblotting and immunocytochemistry protocols to detect GFP and fusion proteins. It detects a ~28 kDa protein that corresponds to GFP in immunoblots of protein extract from cells transfected with, and overexpressing, GFP isoforms. Antibody should be titrated for optimal results in individual sytems.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

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Kathryn A Tworkowski et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(16), 6103-6108 (2008-04-17)
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Chih-Hao Lu et al.
Nature communications, 13(1), 3093-3093 (2022-06-03)
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