NE1013
Anti-PGP9.5 (176-191) Rabbit pAb
liquid, Calbiochem®
Synonyme(s) :
Anti-Neuron Cytoplasmic Protein 9.5, Anti-Ubiquitin Thiolesterase L1, Anti-UCHL1
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
serum
Type de produit anticorps
primary antibodies
Clone
polyclonal
Forme
liquid
Contient
≤0.1% sodium azide as preservative
Espèces réactives
porcine, human, rat, mouse
Fabricant/nom de marque
Calbiochem®
Conditions de stockage
OK to freeze
avoid repeated freeze/thaw cycles
Isotype
IgG
Conditions d'expédition
wet ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... UCHL1(7345)
Catégories apparentées
Description générale
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~26 kDa PGP9.5 protein.
Recognizes the ~26 kDa PGP9.5 protein in mouse dorsal root nerve fibers.
This Anti-PGP9.5 (176-191) Rabbit pAb is validated for use in Immunoblotting, Frozen Sections for the detection of PGP9.5 (176-191).
Immunogène
a synthetic peptide (ASSEDTLLKDAAKVCR) corresponding to amino acids 176-191 of PGP9
Application
Immunoblotting (1:1000)
Frozen Sections (1:2000, fluorescence)
Avertissement
Toxicity: Standard Handling (A)
Forme physique
Undiluted serum.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Remarque sur l'analyse
Positive Control
Mouse dorsal root nerve fibers
Mouse dorsal root nerve fibers
Autres remarques
Caballero, O.L., et al. 2002. Oncogene21, 3003.
Tezel, E., et al. 2000. Clin. Cancer Res.6, 4764.
Tezel, E., et al. 2000. Clin. Cancer Res.6, 4764.
PGP9.5 is also overexpressed in some cancers.
Immunofluorescence protocol
Care should be taken so that the incubation solutions do not evaporate. It is recommended that incubations be performed in a humidified chamber to prevent evaporation. Washing steps should be done in large volumes of phosphate buffered saline (PBS).
1. Warm slides to room temperature.
2. Re-hydrate with PBS for 10-15 min.
3. Remove PBS and incubate in blocking buffer (PBS containing 3% normal donkey serum, 1% bovine serum albumin, 0.3% Triton™ X-100 detergent, and 0.01% NaN3, pH 7.45) for 1 h at room temperature.
4. Dilute primary antibody in blocking buffer. Store diluted antibody at 4°C until use.
5. Remove blocking buffer and add diluted primary antibody.
6. Incubate with primary antibody at 4°C for 18-48 h.
7. Remove primary antibody and wash three times for 10 min with PBS.
8. Dilute secondary antibody in blocking buffer to appropriate dilution (follow manufacture′s recommendation). Store at 4°C until use.
9. Incubate slides in diluted secondary antibody solution for one hour at room temperature.
10. Remove secondary antibody and wash three times for 10 min with PBS.
11. Coverslip slides with appropriate mounting media.
Immunofluorescence protocol
Care should be taken so that the incubation solutions do not evaporate. It is recommended that incubations be performed in a humidified chamber to prevent evaporation. Washing steps should be done in large volumes of phosphate buffered saline (PBS).
1. Warm slides to room temperature.
2. Re-hydrate with PBS for 10-15 min.
3. Remove PBS and incubate in blocking buffer (PBS containing 3% normal donkey serum, 1% bovine serum albumin, 0.3% Triton™ X-100 detergent, and 0.01% NaN3, pH 7.45) for 1 h at room temperature.
4. Dilute primary antibody in blocking buffer. Store diluted antibody at 4°C until use.
5. Remove blocking buffer and add diluted primary antibody.
6. Incubate with primary antibody at 4°C for 18-48 h.
7. Remove primary antibody and wash three times for 10 min with PBS.
8. Dilute secondary antibody in blocking buffer to appropriate dilution (follow manufacture′s recommendation). Store at 4°C until use.
9. Incubate slides in diluted secondary antibody solution for one hour at room temperature.
10. Remove secondary antibody and wash three times for 10 min with PBS.
11. Coverslip slides with appropriate mounting media.
Informations légales
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
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J Nakano et al.
Physiological research, 61(6), 643-647 (2012-10-27)
This study was designed to investigate histological changes in skin tissue accompanying immobilization-induced hypersensitivity. Changes in mechanical sensitivity, epidermal thickness, and peripheral nerve profiles in the upper dermis were examined in glabrous skin of rat hind paw after 1, 2
Antonella S Ríos et al.
Development (Cambridge, England), 149(16) (2022-07-30)
The perception of noxious environmental stimuli by nociceptive sensory neurons is an essential mechanism for the prevention of tissue damage. Etv4 is a transcriptional factor expressed in most nociceptors in dorsal root ganglia (DRG) during the embryonic development. However, its
Masato Ogura et al.
PloS one, 16(2), e0246630-e0246630 (2021-02-05)
Interleukin-31 (IL-31) is involved in excessive development of cutaneous sensory nerves in atopic dermatitis (AD), leading to severe pruritus. We previously reported that PQA-18, a prenylated quinolinecarboxylic acid (PQA) derivative, is an immunosuppressant with inhibition of p21-activated kinase 2 (PAK2)
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