MABS1907

Anti-Ketohexokinase Isoform C Antibody, clone 5F1-3F5

clone 5F1-3F5, from mouse

• Synonyme(s) : EC: 2.7.1.3, Hepatic fructokinase, Fructokinase
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 5F1-3F5, monoclonal
• Espèces réactives: human
• Conditionnement: antibody small pack of 25 μg
• Technique(s): western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_000212
• Numéro d'accès UniProt: P50053-1
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... KHK(3795)

Description

Description générale

Ketohexokinase (UniProt: P50053; also known as EC: 2.7.1.3, Hepatic fructokinase) is encoded by the KHK gene (Gene ID: 3795) in human. Ketohexokinase is a homodimeric cytosolic enzyme in that catalyzes the conversion of fructose to fructose 1 phosphate. It is most abundant in the liver, where it may constitute up to 0.6% of the total liver protein. It has also been reported in kidney, spleen, gut, and pancreas. Very low levels are also found in adrenal, muscle, brain, and eyes. It has multiple fructose binding sites. There are two major isoforms of this enzyme ketofructokinase C and ketofructokinase A. Due to its high Km (~ 28 mM), fructose is preferentially metabolized by the C isoform. Ketohexokinase -C is unique among sugar kinases in that its metabolism of fructose is associated with a rapid depletion of intracellular ATP. Unlike glucokinase, in which excessive phosphorylation of glucose is prevented by a negative feedback system, the metabolism of fructose by ketohexokinase-C results in rapid phosphorylation with a fall in intracellular phosphate and ATP. If the ATP levels fall significantly then the protein synthesis is transiently stopped and cells may develop features that are consistent with ischemia. Mutations in KHK gene has been linked to fructosuria, a clinically benign condition that is characterized nu the incomplete metabolism of fructose in the liver, leading to its urinary excretion. (Ref.: Diggle, CP et al., (2009). J. Histochem. Cytochem. 57(8): 763-774; Hayward, BE., and Bonthron, DT (1998). Eur. J. Biochem. 257(1); 85-91).

Spécificité

Clone 5F1-3F5 specifically detects isoform C of human ketohexokinase.

Immunogène

A full-length human recombinant Ketohexokinase, isoform C.

Application

Anti-Ketohexokinase Isoform C, clone 5F1-3F5, Cat. No. MABS1907, is a mouse monoclonal antibody that detectsKetohexokinase and has been tested for use in Western Blotting.

Research Category
Signaling

Western Blotting Analysis: 2 µg/mL from a represenative lot detected Ketohexokinase Isoform C in HEK293 cells expressing HA-tagged KHK-C.

Western Blotting Analysis: 1 µg/mL from a represenative lot detected Ketohexokinase Isoform C in human liver tissue lysate.

Qualité

Evaluated by Western Blotting in human kidney tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected Ketohexokinase Isoform C in human kidney tissue lysate.

Description de la cible

~33 kDa observed; 32.52 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable