MABN2434M

Anti-Retinoschisin Antibody, clone 3R10

clone 3R10, from mouse

• Synonyme(s) : X-linked juvenile retinoschisis protein, RS1-3R10
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 3R10, monoclonal
• Espèces réactives: human, mouse
• Conditionnement: antibody small pack of 25 μg
• Technique(s): immunofluorescence: suitable; western blot: suitable
• Isotype: IgG2aκ
• Numéro d'accès NCBI: NP_000321
• Numéro d'accès UniProt: O15537
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... RS1(6247)

Description

Description générale

Retinoschisin (UniProt: O15537; also known as X-linked juvenile retinoschisis protein) is encoded by the RS1 (also known as XLRS1) gene (Gene ID: 6247) in human. Retinoschisin is discoidin domain-containing protein that is localized along the extracellular surfaces of rod and cone photoreceptors and bipolar cells including the synapses. It is undetectable in the inner plexiform layers and the inner nuclear layer. It is active in cell adhesion process during retinal development and maintains the cellular organization and synaptic structure of the retina. Retinoschisin is a homooctamer of 4 homodimers that are linked by disulfide bonds. It contains a single F5/8 type C domain. Deleterious mutations in RS1 encoding retinoschisin are associated with X-linked juvenile retinoschisis (RS), a common form of macular degeneration in males, which is characterized by a negative electroretinogram pattern and by a splitting of the inner retina. Approximately half of cases of X-linked retinoschisis have bilateral peripheral retinoschisis in the inferotemporal part of the retina. Retinoschisin levels are shown to be up-regulated during the differentiation of a retinoblastoma cell line.

Spécificité

Clone 3R10 detects Retinoschisin in murine retinal cells. It targets an epitope within 18 amino acids from the N-terminal region.

Immunogène

GST-tagged fusion protein containing a the LSSTEDEGEDPWYQKAC peptide fragment corresponding to amino acids 22-39 from the N-terminal region of human Retinoschisin precusor protein.

Application

Anti-Retinoschisin, clone 3R10, Cat. No. MABN2434, is a highly specific mouse monoclonal antibody that targets Retinoschisin and has been tested in Immunofluorescence and Western Blotting..

Immunofluorescence Analysis: A representative lot detected Retinoschisin in Immunofluorescence applications (Min, S.H., et. al. (2005). Mol Ther. 12(4):644-51; Weber, B.H., et. al. (2002). Proc Natl Acad Sci USA. 99(9):6222-7).

Western Blotting Analysis: A representative lot detected Retinoschisin in Western Blotting applications (Min, S.H., et. al. (2005). Mol Ther. 12(4):644-51; Wu, W.W., et. al. (2003). J Biol Chem. 278(30):28139-46).

Research Category
Neuroscience

Qualité

Evaluated by Western Blotting in mouse retinal tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected Retinoschisin in 10 µg of mouse retinal tissue lysate.

Description de la cible

~23 kDa observed; 25.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1