FCMAB180F

Milli-Mark^® Anti-CD13-FITC Antibody, clone WM-47

clone WM-47, Milli-Mark^®, from mouse

• Synonyme(s) : Aminopeptidase N (AP-N), hAPN, Alanyl aminopeptidase, Microsomal aminopeptidase, Aminopeptidase M (AP-M), gp150, Myeloid plasma membrane glycoprotein CD13
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.44

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Conjugué: FITC conjugate
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: WM-47, monoclonal
• Espèces réactives: human
• Fabricant/nom de marque: Milli-Mark^®
• Technique(s): flow cytometry: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_001141.2
• Numéro d'accès UniProt: P15144
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ANPEP(290)

Description

Description générale

CD13 is a 150 kDa Type II integral membrane protein composed of 967 amino acids (Turni, 2002; Ashmun, 1995; Look, 1989). CD13 is identical in structure to aminopeptidase N, a membrane-bound zinc-binding metalloprotease (Turni, 2002). CD13 is expressed on the surface of committed granulocyte-monocyte progenitors (CFU-GM) and by cells of the granulocyte and monocyte lineages at all stages of differentiation (Turni, 2002; Ashmun, 1995; Look, 1989). The antigen is also expressed on a small proportion of large granular lymphocytes, but not on B or T cells (Ashmun, 1995; Look, 1989). Further, CD13 is expressed on some non-hematopoietic cells including fibroblasts, endothelial cells, epithelial cells from renal proximal tubules and intestinal brush border, bone marrow stromal cells, osteoclasts, and cells lining the bile duct canaliculi (Turni, 2002; Ashmun, 1995; Look, 1989).
Anti-CD13, WM-47, was included in the Fourth and Fifth International Workshop and Conference on Leucocyte Differentiation Antigens and studies by a number of laboratories confirmed its reactivity with CD 13 (Ashmun, 1995; Look, 1989).

Spécificité

Antibody recognizes Human CD13 antigen.

Immunogène

Leukaemic cells from a case of chronic myeloid leukaemia (CML).

Application

Detect CD13 using this Milli-Mark Anti-CD13-FITC Antibody, clone WM-47 validated for use in FC.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Qualité

Evaluated by flow cytometry using human PBMCs

Description de la cible

150 kDa (Turni, 2002; Ashmun, 1995; Look, 1989)

Forme physique

Protein A purified

Purified Mouse monoclonal IgG1 conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.

Stockage et stabilité

Maintained refrigerated at 2-8°C from date of receipt. Protected from light in undiluted aliquots for 6 months from date of receipt.

Remarque sur l'analyse

Control
PBMCs

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable