AB5935
Anti-Tyrosine Hydroxylase Antibody, phosphoSer 40
Chemicon®, from rabbit
Synonyme(s) :
Tyrosine Monooxygenase
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Produit purifié par
affinity chromatography
Espèces réactives
avian, rat, mouse
Réactivité de l'espèce (prédite par homologie)
quail (100% sequence homology), nonhuman primates (100% sequence homology), human (100% sequence homology), porcine (100% sequence homology)
Fabricant/nom de marque
Chemicon®
Technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Modification post-traductionnelle de la cible
phosphorylation (pSer40)
Catégories apparentées
Description générale
Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
Spécificité
Other species have not been tested.
Tyrosine Hydroxylase, phosphoSer40. The antibody recognizes a protein of 60 kDa corresponding to TH phosphorylated at Ser40. Depending on brain region being studied, protein loads and detection methods used some higher molecular weight bands may be detected.
Immunogène
Epitope: phospho-Ser40
Synthetic peptide from the phosphoSer40 of rat Tyrosine Hydroxylase.
Application
Anti-Tyrosine Hydroxylase Antibody, phosphoSer 40 detects level of Tyrosine Hydroxylase & has been published & validated for use in IC, IH & WB.
Immunohistochemistry:
A 1:1,000 dilution of a previous lot was used in IH.
Immunocytochemistry:
A 1:1,000 dilution of a previous lot was used in IC.
Optimal working dilutions must be determined by the end user.
A 1:1,000 dilution of a previous lot was used in IH.
Immunocytochemistry:
A 1:1,000 dilution of a previous lot was used in IC.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
Qualité
Routinely evaluated by Western Blot on PC12 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected TYROSINE HYDROX PSER40 on 10 μg of PC12 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected TYROSINE HYDROX PSER40 on 10 μg of PC12 lysates.
Description de la cible
60 kDa
Forme physique
ImmunoAffinity Purified
Purified rabbit polyclonal liquid in buffer containing 10mM Hepes, pH 7.5, 150mM NaCl, 100 µg/mL BSA, and 50% glycerol.
Stockage et stabilité
Stable for 6 months at -20°C in undiluted from date of receipt.
Remarque sur l'analyse
Control
POSITIVE CONTROL: brain (sympathetic nerve terminals) and adrenal glands.
POSITIVE CONTROL: brain (sympathetic nerve terminals) and adrenal glands.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Vous ne trouvez pas le bon produit ?
Essayez notre Outil de sélection de produits.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Zhenqiang Zhao et al.
Frontiers in cellular neuroscience, 10, 291-291 (2017-01-10)
Mouse embryonic fibroblasts (MEFs) and human foreskin fibroblasts (HFFs) are used for the culture of human embryonic stem cells (hESCs). MEFs and HFFs differed in their capacity to support the proliferation and pluripotency of hESCs and could affect cardiac differentiation
Luis M Colon-Perez et al.
Neuropharmacology, 137, 178-193 (2018-05-08)
Among cathinone drugs known as bath salts, methylenedioxypyrovalerone (MDPV) exerts its potent actions via the dopamine (DA) system, and at intoxicating doses may produce adverse behavioral effects. Previous work by our group suggests that prolonged alterations in correlated neural activity
Tsung-Jung Ho et al.
Evidence-based complementary and alternative medicine : eCAM, 2017, 5642708-5642708 (2017-04-13)
Methamphetamine (METH) is a major drug of abuse worldwide, and no efficient therapeutic strategies for treating METH addiction are currently available. Continuous METH use can cause behavioral upregulation or psychosis. The dopaminergic pathways, particularly the neural circuitry from the ventral
Cristina Núñez et al.
Endocrinology, 150(7), 3118-3127 (2009-01-31)
Chronic opiate exposure induces neurochemical adaptations in the noradrenergic system. Enhanced responsiveness of the hypothalamo-pituitary-adrenal axis after morphine withdrawal has been associated with hyperactivity of ascending noradrenergic input from the nucleus of the solitary tract (NTS-A(2)) cell group to the
Gregory V Carr et al.
Frontiers in pharmacology, 8, 666-666 (2017-10-24)
Pharmacological studies indicate that dopamine D1-like receptors (D1 and D5) are critically involved in cognitive function. However, the lack of pharmacological ligands selective for either the D1 or D5 receptors has made it difficult to determine the unique contributions of
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique