71493-M

HRV 3C Protease

Highly efficient fusion tag removal at low temperature

• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.54

Propriétés

• Niveau de qualité: 400
• Fabricant/nom de marque: Novagen^®
• Conditions de stockage: OK to freeze
• Concentration: 1.8-3.0 unit/μL
• Conditions d'expédition: dry ice; wet ice
• Température de stockage: −20°C

Description

Description générale

Recombinant type 14 3C protease from human rhinovirus (HRV 3C) is a recombinant restriction-grade protease. HRV 3C is a highly purified recombinant 6XHis-fusion protein, that recognizes the same cleavage site as the native enzyme: LeuGluValLeuPheGln?GlyPro. The small, 22-kDa size of the protease, optimal activity at 4°C, high specificity, and His Tag^® fusion make HRV 3C protease an ideal choice for rapid removal of fusion tags. The pET expression vectors pET-47b(+) through pET-50b(+) incorporate the HRV 3C protease cleavage site in combinations withHis Tag^®, S•Tag, thioredoxin (Trx•Tag), glutathione-S-transferase (GST•Tag), and NusA (Nus•Tag) sequences. The combination of pET-47b(+) to pET-50b(+) vectors for expression, HRV 3C protease for fusion tag cleavage, and Ni-NTA His•Bind metal affinity chromatography for protein purification as well as protease and fusion tag removal allows production of recombinant proteins free of vector-encoded sequences. Other expression vectors, including the pET-52b(+) vector, the InsectDirect pIEx-9 vector, and the multisystem pTriEx-6 vector, all incorporate the HRV 3C protease cleavage site in combination with the Strep•Tag II and His•Tag sequences. Supplied as: 1.8-3.0 U/µl in 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM EDTA, 0.5 mM THP, and 50% glycerol.

Application

• One unit will cleave >95% of 100 µg His•Tag fusion control protein in 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 at 4°C for 16 h.

Composants

• 500 U - HRV 3C Protease
• 10 µg - HRV 3C Cleavage Control Protein
• 10 ml - 10X HRV 3C Cleavage Buffer

Avertissement

Toxicity: Standard Handling

Forme physique

In 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM EDTA, 0.5 mM THP, and 50% glycerol.

Informations légales

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable