70856
RNase A Solution
Convenient solution for selective degradation of RNA
Synonyme(s) :
RNase A Enzyme
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About This Item
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.54
Produits recommandés
Forme
solution
Fabricant/nom de marque
Novagen®
Conditions de stockage
OK to freeze
Concentration
10 mg/mL
Technique(s)
DNA purification: suitable
Conditions d'expédition
ambient
Température de stockage
10-30°C
Description générale
RNase A Solution is a convenient alternative to powdered RNase A. It is a highly purified preparation of bovine pancreatic ribonuclease A suitable for use in selective removal of RNA. It has been pretreated to remove DNase I and is suitable to remove RNA contamination during plasmid and genomic DNA purification procedures. Supplied at a concentration of 10 mg/ml in 10 mM Tris-HCl, 1 mM EDTA, 50% glycerol, pH 7.5.
Application
RNase A Solution has been used:
- in cell cycle profiling of embryonic mouse neuroectodermal cells (NE-4C)
- in the digestion of RNA for extraction of DNA from human osteosarcoma cell lines for chromatin immunoprecipitation (ChIP) assays
- to treat control adult and embryonic thymi cells during staining
- to remove RNA from affibody–mRNA fusion clones before clone screening
Actions biochimiques/physiologiques
RNaseA is an endoribonuclease that catalyzes the degradation of phosphodiester linkages with a pyrimidine base at the 3′ -position on single-stranded RNA via, transphosphorylation and hydrolysis mechanism.
Avertissement
Toxicity: Standard Handling (A)
Informations légales
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
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