69182

DNase I, RNase free

For applications in which maintenance of RNA integrity is critical

• Synonyme(s) : RNase-free DNase I
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.85

Propriétés

• Forme: liquid
• Fabricant/nom de marque: Novagen^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles
• Technique(s): DNA extraction: suitable
• Adéquation: suitable for nucleic acid purification
• Application(s): diagnostic assay manufacturing
• Conditions d'expédition: wet ice
• Température de stockage: −20°C

Description

Description générale

RNase-free DNase I digests either single- or double-stranded DNA, producing a mixture of mono- and oligonucleotides. Purified to be free of RNase, this preparation is qualified for applications in which maintenance of the integrity of RNA is critical. The enzyme selectively degrades DNA in the presence of RNA and can be used to remove DNA template following in vitro transcription reactions. This enzyme is also useful in other applications such as DNase footprinting and nick translation.

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

One unit will degrade 1 μg DNA in 10 minutes at 37°C. The reaction mixture (50 µl) contains 80 mM HEPES pH 7.5, 10 mM NaCl, 5 mM MgCl₂, 10 mM DTT, 1 µg plasmid DNA, and enzyme.

Informations légales

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable