553506-M
RAP, Human, Recombinant, E. coli
Synonyme(s) :
Receptor Associated Protein
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About This Item
Code UNSPSC :
12352202
Produits recommandés
Pureté
≥90% (SDS-PAGE)
Niveau de qualité
Forme
liquid
Fabricant/nom de marque
Calbiochem®
Conditions de stockage
OK to freeze
avoid repeated freeze/thaw cycles
Température de stockage
−70°C
Description générale
Recombinant, human RAP fused to GST, expressed in E. coli, and subsequently cleaved with thrombin to remove GST. Antagonizes the ligand binding of certain members of the low-density lipoprotein receptor family such as low density lipoprotein receptor-related protein (LRP) and very low-density lipoprotein (VLDL) receptor. Promotes the proper folding and subsequent exocytic trafficking of the wild-type low density lipoprotein receptor (LDLR) and several of its class 2 mutants. Expressed as a GST-fusion protein that is subsequently cleaved with thrombin. Typical concentrations to block LRP or VLDL receptor are 200-300 nM. For antagonism of LDLR, 1 µM is recommended.
Recombinant, human RAP fused to GST, expressed in E. coli, and subsequently cleaved with thrombin to remove GST. Receptor Associated Protein (RAP) antagonizes the ligand binding of certain members of the low-density lipoprotein receptor family such as low-density lipoprotein receptor-related protein (LRP) and very low density lipoprotein (VLDL) receptor. Promotes the proper folding and subsequent exocytic trafficking of the wild-type low-density lipoprotein receptor (LDLR) and several of its class 2 mutants. Typical concentrations used to block LRP or VLDL receptor are 200-300 nM; for antagonism of LDLR, 1 µM is recommended.
Avertissement
Toxicity: Standard Handling (A)
Forme physique
150 mM NaCl, 100 mM Tris-HCl, pH 7.4.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Autres remarques
Li, Y., et al. 2002. Biochemistry41, 4921.
Salicioni, A.M., et al. 2002. J. Biol. Chem.277, 16160.
Anderson, O.M., et al. 2001. Biochemistry40, 15408.
Bu, G. 2001. Int. Rev. Cytol.209, 79.
Williams, S.E., et al. 1992. J. Biol. Chem.267, 9035
Salicioni, A.M., et al. 2002. J. Biol. Chem.277, 16160.
Anderson, O.M., et al. 2001. Biochemistry40, 15408.
Bu, G. 2001. Int. Rev. Cytol.209, 79.
Williams, S.E., et al. 1992. J. Biol. Chem.267, 9035
Informations légales
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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