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Sigma-Aldrich

Ras Activation Assay Kit

Synonyme(s) :

Ras Activity Assay

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About This Item

Code UNSPSC :
12161503
eCl@ss :
32161000
Nomenclature NACRES :
NA.84

Fabricant/nom de marque

Upstate®

Niveau de qualité

100

Technique(s)

activity assay: suitable

Numéro d'accès NCBI

NM_001085049.1
NM_012219.3

Numéro d'accès UniProt

O14807

Conditions d'expédition

dry ice

Informations sur le gène

human ... MRAS(22808)

Description générale

Muscle RAS oncogene homolog (MRAS) plays a key role in cardiovascular diseases. The MRAS gene codes for a member of the ras superfamily of GTP-binding proteins. MRAS is found in all tissues, particularly in the cardiovascular system. It is involved in signal transduction, including cell growth and differentiation.

Conditionnement

Kit capacity: 30 assays

Composants

Ras Assay Reagent (Raf-1 RBD, agarose) (Cat.# 14-278)

Anti-Ras, clone RAS10 (Cat.# 05-516)

100X GTPγS, 10mM (Cat.# 20-176)

100X GDP, 100mM (Cat.# 20-177)

Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)

Qualité

This kit precipitated Ras-GTP from HeLa cell lysates that had been loaded with GTPγS. The precipitated Ras-GTP was detected by western immunoblot using 1μg/ml anti-Ras, clone RAS10

Liaison

Replaces: SGT435

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictogrammes

CorrosionEnvironment
GHS05,GHS09

Mention d'avertissement

Danger

Mentions de danger

H318,H410

Classification des risques

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1

Code de la classe de stockage

10 - Combustible liquids

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AnhCo Nguyen et al.
Molecular and cellular biology, 22(9), 3035-3045 (2002-04-10)
While scaffold proteins are thought to be key components of signaling pathways, their exact function is unknown. By preassembling multiple components of signaling cascades, scaffolds are predicted to influence the efficiency and/or specificity of signaling events. Here we analyze a
Richard J Calvert et al.
Analytical biochemistry, 343(2), 283-292 (2005-07-16)
Accurate measurement of activity of wild-type K-ras protein is important due to its tumor suppressor action in tissues such as lung. A published method by Taylor and co-workers uses plasmid-containing Escherichia coli cells to produce a glutathione-S-transferase/raf-1 ras binding domain
Shin-ichiro Miura et al.
Biochemical and biophysical research communications, 306(4), 924-929 (2003-06-25)
Since we recently reported that high density lipoprotein, which contains the bioactive lipid sphingosine-1-phosphate (S1P) [Arterioscler. Thromb. Vasc. Biol. 23 (2003) 802], induced human coronary artery endothelial cell (HCEC) tube formation mediated by a Ras/Raf/ERK (extracellular signal-activated kinase) pathway, we
Michael R Bennett et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 23(18), 7207-7217 (2003-08-09)
Neurofibromatosis type 1 (NF1) patients are predisposed to learning disabilities, macrocephaly, and brain tumors as well as abnormalities on magnetic resonance imaging that are postulated to result from abnormal myelination. Here we show that Nf1+/- spinal cords in adult mice
X Shen et al.
The Biochemical journal, 352 Pt 3, 913-919 (2000-12-06)
Interleukin 1beta (IL-1beta) suppresses the IL-6-dependent induction of type II acute-phase response genes, but the underlying mechanism for this suppression remains uncertain. Here we report that treatment of human hepatocullular carcinoma HepG2 cells with IL-1beta inhibited the IL-6-dependent binding of
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