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Merck

17-128

Alkaline/Acid Phosphatase Assay Kit (R-R-A-pS-V-A)

Alkaline/Acid Phosphatase Assay Kit is routinely used to detect phosphatase activity by either dephosphorylation of the phosphopeptide (RRApSVA) or hydrolysis of pNPP.

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UNSPSC Code:
12161503
NACRES:
NA.84
eCl@ss:
32161000
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Quality Level

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (phosphatase)

detection method

colorimetric

shipped in

wet ice

Application

Used to detect/quantify: Alkaline/Acid

Packaging

Kit capacity: 100 assays

Analysis Note

routinely used to detect phosphatase activity by either dephosphorylation of the phosphopeptide (RRApSVA) or hydrolysis of pNPP

Other Notes

Malachite Green Solution A (Cat.# 20-105)

Malachite Green Additive (Cat.# 20-104)

Phosphate Standard (Cat.# 20-103)

Serine Phosphopeptide (RRApSVA) (Cat.# 12-220)

pNPP (p-Nitrophenyl Phosphate) (Cat.# 20-106)

NiCl2, 40mM (Cat.# 20-178)

pNPP Ser/Thr Assay Buffer (Cat.# 20-179)

96-well microtiter plate

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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Danger

Hazard Classifications

Aquatic Chronic 3 - Carc. 1A Inhalation - Met. Corr. 1 - Repr. 1B - Skin Sens. 1 - STOT RE 2

target_organs

Lungs

Classe de stockage

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 3



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Quantification of subnanomolar amounts of phosphate bound to seryl and threonyl residues in phosphoproteins using alkaline hydrolysis and malachite green.
Ekman, P and Jager, O
Analytical biochemistry, 214, 138-141 (1993)
Nathan J Castro et al.
Tissue engineering. Part A, 22(13-14), 940-948 (2016-06-15)
Osseous tissue defects caused by trauma present a common clinical problem. Although traditional clinical procedures have been successfully employed, several limitations persist with regards to insufficient donor tissue, disease transmission, and inadequate host-implant integration. Therefore, this work aims to address
An investigation of the substrate specificity of protein phosphatase 2C using synthetic peptide substrates; comparison with protein phosphatase 2A
Donella Deana, A., et al
Biochimica et Biophysica Acta, 1051, 199-202 (1990)



Numéro d'article de commerce international

RéférenceGTIN
17-12804053252475177