14-840-M
AMPK (α1, β1, γ1) Protein, active, 10 µg
Active, recombinant, human AMPK (α1, β1, γ1) holoenzyme, comprised of full length N-terminal 6His-tagged AMPKα1, full length untagged AMPKβ1 & full length untagged AMPKγ1. For use in Kinase Assays.
Synonyme(s) :
AMPK kinase
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About This Item
Code UNSPSC :
12352202
eCl@ss :
32160405
Nomenclature NACRES :
NA.32
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Source biologique
human
Niveau de qualité
Produit recombinant
expressed in E. coli
Forme
liquid
Durée de conservation
6 mo.
Poids mol.
Mw 30 kDa (AMPKß1)
Mw 38 kDa (AMPKγ1)
Mw 64 kDa (AMPKα1)
Fabricant/nom de marque
Upstate®
Technique(s)
activity assay: suitable (kinase)
Solubilité
water: soluble
Numéro d'accès GenBank
Température de stockage
−70°C
Informations sur le gène
human ... PRKAB1(5564)
Description générale
Research area: Cell Signaling
Product Source: Expressed in E. coli cells and activated in vivo.
Protein Target: AMPK (α1, β1, γ1)
Target Sub-Family: CAMK
Recombinant, human AMPK (α1, β1, γ1) holoenzyme, comprised of full- length N-terminal 6His-tagged AMPKα1, full length untagged AMPKβ1 and full length untagged AMPKγ1. The AMPK α catalytic subunit in mammals is encoded by α1 and α2, two β genes β1 and β2 and three γ subunit genes γ1, γ2, and γ3.
Product Source: Expressed in E. coli cells and activated in vivo.
Protein Target: AMPK (α1, β1, γ1)
Target Sub-Family: CAMK
Recombinant, human AMPK (α1, β1, γ1) holoenzyme, comprised of full- length N-terminal 6His-tagged AMPKα1, full length untagged AMPKβ1 and full length untagged AMPKγ1. The AMPK α catalytic subunit in mammals is encoded by α1 and α2, two β genes β1 and β2 and three γ subunit genes γ1, γ2, and γ3.
Application
For use in Kinase Assays.
Research Category
Metabolism
Apoptosis & Cancer
Metabolism
Apoptosis & Cancer
Research Sub Category
Diabetes
Obesity
Metabolic Disorders
Diabetes
Obesity
Metabolic Disorders
Actions biochimiques/physiologiques
Under conditions of nutrient scarcity, AMPK functions as a metabolic checkpoint and inhibits cellular growth. Upon activation, AMPK promotes glucose uptake and fatty acid oxidation thereby reducing energy-consuming processes like protein and lipid synthesis. Furthermore, AMPK regulates autophagy and mitophagy. It also facilitates catabolic pathways to generate more ATP as a response to decreased ATP production.
Conditionnement
Also available in 250µg size as catalog # 14-840M - Call for pricing and availability.
Qualité
Routinely evaluated by phosphorylation of substrate peptide
Forme physique
Cobalt-agarose
Stockage et stabilité
6 months at -70°C
Autres remarques
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Informations légales
GenBank is a registered trademark of United States Department of Health and Human Services
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Skin Sens. 1
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
AMPK and the biochemistry of exercise: Implications for human health and disease
Richter EA and Ruderman NB
The Biochemical Journal, 418(2), 261?275-261?275 (2009)
AMPK: guardian of metabolism and mitochondrial homeostasis
Herzig S and Shaw RJ
Molecular Cell Biology, 19, 121?135-121?135 (2018)
The AMP-activated protein kinase (AMPK) signaling pathway coordinates cell growth, autophagy, & metabolism
Mihaylova MM and Shaw RJ
Nature Cell Biology, 13(9), 1016?1023-1016?1023 (2011)
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