The Weigert's Iron Hematoxylin (1159730002) is typically used on either FFPE (formalin-fixed paraffin-embedded tissue) or fresh frozen tissue that has been fixed prior to staining with Weigert's Iron Hematoxylin. Since the solution is not osmotically balanced, the working solution is not intended for use on unfixed tissue. It is note that fixation with either formalin or Bouin's Solution and do not mention staining unfixed tissue. Although the IFU does not specifically mention fresh frozen tissue that has been sectioned on a cryostat and fixed prior to staining, Weigert's should be able to stain fixed tissue specimens.
1.15973
Weigert′s iron hematoxylin kit
for nuclear staining in histology
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NACRES:
NA.41
UNSPSC Code:
41116124
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IVD
for in vitro diagnostic use
dilution
(for nuclear staining in histology)
application(s)
clinical testing
cytology
diagnostic assay manufacturing
hematology
storage temp.
15-25°C
General description
Weigert′s iron hematoxylin kit - for nuclear staining in histology is a ready to use staining kit used for human-medical cell diagnosis and serves the purpose of the histological investigation of sample material of human origin, for example histological sections of e. g. the liver, the kidney, the intestine, the placenta and similar. Aluminium hematoxylins (e. g. nuclear fast red aluminium sulfate) are washed out of the tissue sections by acidic staining solutions. Iron hematoxylin solutions (e. g. Weigert′s iron hematoxylin), on the other hand, are acid-resistant and are preferentially used for staining nuclei in histological tissue sections in which counterstaining is done with acidic staining solutions. Examples here include the visualization of elastic fibers according to the van Gieson method (e. g. Elastica van Gieson (Product number 1.15974)) and the various trichromo connective-tissue staining methods (e. g. according to Masson-Goldner (Product number 1.00485). Commonly used hemalum solutions such as Mayer′s Hemalum, Delafield, Gill and Harris tend to procedure also weak nucleic staining only when used with acidic counter-stains.The kit contains two solutions; an alcoholic hematoxylin solution and a hydrochloric acid iron(III)nitrate solution, which are ready-to-use and should be applied at 3-5 μm paraffin sections.
The package is sufficient for 400 - 500 applications. Weigert′s Iron hematoxylin kit is an IVD & CE product and can be used in the clinical laboratory for diagnostics. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
The package is sufficient for 400 - 500 applications. Weigert′s Iron hematoxylin kit is an IVD & CE product and can be used in the clinical laboratory for diagnostics. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
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Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1
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3 - Flammable liquids
WGK
WGK 1
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Is it possible to use 1159730002 on fresh tissue?
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Can Weigert's Iron Hematoxylin be used to demonstrate iron in bone marrow smears?
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The Weigert's Hematoxylin Iron solutions are intended for nuclear staining and are less prone to wash out of cells/tissues. These kits are suitable for staining bone marrow smears, but caution is advised when decalcifying bone marrow sections, as harsh acids may remove the iron from the tissue.
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