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A propos de cet article
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IP, WB
Citations:
32
Service technique
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rabbit
Quality Segment
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse, Xenopus, pig, rat
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... PPP2R1A(5518)
General description
63-65kDa
Immunogen
KLH-conjugated, synthetic peptide of 13 amino acids (MAAADGDDSLY-Ahx-C), containing amino acids 1-11 of human PP2A, A subunit. Ahx is aminohexanoic acid. The immunizing sequence is conserved in human, mouse, rat, porcine and Xenopus.
Application
Research Sub Category
Synapse & Synaptic Biology
Neurodegenerative Diseases
Synapse & Synaptic Biology
Neurodegenerative Diseases
Anti-PP2A Antibody, A subunit detects level of PP2A & has been published & validated for use in IP & WB.
Biochem/physiol Actions
PP2A A subunit, α isoform
Physical form
100mM triethylamine, 100mM sodium phosphate, 0.02% sodium azide before the addition of glycerol to 30%.
Analysis Note
Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Routinely evaluated by immunoblot on RIPA lysates from 3T3/A31 cells.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Classe de stockage
12 - Non Combustible Liquids
wgk
WGK 1
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