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Key Documents

MRC-5 SV2

84100401, human lung (fetal), Fibroblast

Synonym(s):

MRC-5V2 Cells, MRC5V2 Cells

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About This Item

UNSPSC Code:
41106514

product name

MRC-5 SV2, 84100401

biological source

human lung (fetal)

growth mode

Adherent

karyotype

Modal no. = 60-80, hyperdiploid

morphology

Fibroblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human foetal lung, SV40 transformed

Cell Line Description

Derived from the parental cell line MRC-5 by transformation with the SV40 virus. The cells do not senesce.

Application

Transformation and virus studies: SV40

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 11
D5S818: 11,12
D7S820: 10
THO1: 8
TPOX: 8
vWA: 15

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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