UHPLC/MS Analysis of Diazepam and Metabolites on Titan C18, Comparison of Flow Rate and Column Length
Matériaux
Étalon
Réf. du produit
Description
Tarif
Oxazepam solution
1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Nordiazepam solution
1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Diazepam solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Temazepam solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Titan C18, 2 cm x 2.1 mm I.D., 1.9 μm particles (577120-U)
mobile phase
[A] 0.1% formic acid; [B] acetonitrile; (60:40, A:B)
flow rate
various
temp.
35 °C
detector
TIC MRM (UV also runs in series)
injection
1 μL
sample
100 ng/mL in mobile phase
Description
Remarque sur l'analyse
These two sets of chromatograms show separations on 100, 50, 30, and 20 mm x 2.1 mm I.D. columns and at different flow rates and demonstrate the utility of the 1.9 μm Titan particles. The highest grade solvents provided clean, robust operation. Cerilliant CRMs provided reliable quantification.
Informations légales
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