HPLC Analysis of Flavanone Enantiomers on Astec^® CYCLOBOND I 2000 DMP, Ethanol:Hexane Mobile Phase

column

CYCLOBOND I 2000 DMP, 25 cm x 4.6 mm I.D., 5 μm particles (20724AST)

mobile phase

[A] ethanol; [B] hexane; (20:80, A:B)

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 254 nm

injection

1 μL

sample

1 mg/mL in methanol