- Disulfide bonds are required for Serratia marcescens nuclease activity.
Disulfide bonds are required for Serratia marcescens nuclease activity.
Nucleic acids research (1992-10-11)
T K Ball, Y Suh, M J Benedik
PMID1329033
RÉSUMÉ
The role of the two disulfide bonds found in the Serratia marcescens nuclease were tested by site directed mutagenesis and were found essential for nuclease activity, although slight residual activity remained. The requirement for disulfide bond formation may play a role in preventing the lethal action of nuclease while in the bacterial cytoplasm.
MATÉRIAUX
Référence du produit
Marque
Description du produit
Millipore
Nucléase Benzonase®, ≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution
Sigma-Aldrich
Nucléase Benzonase®, ultrapure, ≥250 units/μL, ≥99% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution, ultrapure grade