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V8137

Sigma-Aldrich

Anti-V5 antibody produced in rabbit

IgG fraction of antiserum

Synonyme(s) :

Rabbit Anti-V5

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About This Item

Numéro MDL:
MFCD03456076
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.56

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

IgG fraction of antiserum

Type de produit anticorps

primary antibodies

Clone

polyclonal

Technique(s)

immunocytochemistry: 5 μg/mL using V5 tagged fusion protein in methanol-acetone fixed transiently transfected 293T cells
immunoprecipitation (IP): 5 μg/mL using V5 tagged fusion protein from extracts of transfected cells
western blot: 2.5 μg/mL using V5 tagged fusion protein expressed in whole extracts of transfected cells

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Description générale

V5 is an amino coterminal protein consisting of a C-terminus cysteine-rich region that resembles a cysteine-rich metal binding domain. It is encoded by the "P" gene of the paramyxovirus SV5.
The rabbit Anti-V5 synthetic peptide antibody reacts with V5 tagged recombinant fusion proteins expressed in transfected mammalian cells and from in vitro translation.

Spécificité

Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins

Application

Anti-V5 antibody produced in rabbit has been used in:
  • immunocytochemistry
  • immunoprecipitation
  • western blot

Forme physique

Solution in 0.01M phosphate buffered saline, pH 7.4 containing 15 mM sodium azide.

Stockage et stabilité

Store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Journal of virology, 88(24), 14326-14339 (2014-10-03)
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