SRP4693

Apolipoprotein A−I human

recombinant, expressed in E. coli, ≥97% (SDS-PAGE), ≥97% (HPLC)

• Synonyme(s) : APOA1, Apo-AI, Apolipoprotein A-I, C117399, MGC117399
• Numéro CAS: 1426185-50-3
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in E. coli
• Essai: ≥97% (HPLC); ≥97% (SDS-PAGE)
• Forme: lyophilized
• Poids mol.: ~28 kDa
• Conditionnement: pkg of 100 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Impuretés: endotoxin, tested
• Numéro d'accès NCBI: NM_000039
• Numéro d'accès UniProt: P02647
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: human ... ApoA1(335)

Description

Description générale

ApoA-I (apolipoprotein A-I) is a 29.0kDa protein produced in the liver and intestine, and secreted as the predominant constituent of nascent high-density lipoprotein (HDL) particle.[1][2] Recombinant human ApoA-I is a 28.2kDa protein of 244 amino acid residues.

ApoA-I, which is found exclusively in HDL, has a unique ability to capture and solubilize free cholesterol. This apoA-I ability enables HDL to remove excess peripheral cholesterol and return it to the liver for recycling and excretion. This process, called reverse cholesterol transport, is though to inhibit atherogenesis. For this reason HDL is also known as the "good cholesterol." The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I (called apoA-I Milano). The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases.

Application

Apolipoprotein A-I human has been used in the cholesterol efflux assay.[3][4]

Actions biochimiques/physiologiques

ApoA-I (apolipoprotein A-I), which is found exclusively in HDL (high-density lipoprotein), has a unique ability to capture and solubilize free cholesterol.[5] This apoA-I ability enables HDL to remove excess peripheral cholesterol and return it to the liver for recycling and excretion.[6] This process, called reverse cholesterol transport, is thought to inhibit atherogenesis.[7] For this reason HDL is also known as the "good cholesterol." The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I (called apoA-I Milano). The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases.[8] Changes in the level of serum apoA-I may serve as a prognostic marker for non-metastatic nasopharyngeal carcinoma.[9] Low levels of apoA-I in the plasma is linked to hyperhomocysteinemia.[10]

Forme physique

Sterile filtered and Lyophilized without additives.

Notes préparatoires

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitution

Reconstitute in water to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4 °C for 1 week or –20 °C for future use.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable