SHC004
MISSION® pLKO.1-puro TurboGFP™ shRNA Control Plasmid DNA
shRNA sequence targeting tGFP
Synonyme(s) :
MISSION® Control Vectors
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About This Item
Produits recommandés
Niveau de qualité
Gamme de produits
MISSION®
Concentration
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
Conditions d'expédition
dry ice
Température de stockage
−20°C
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Description générale
The MISSION TurboGFP shRNA Control Vector is a 7,087 base pair lentivirus plasmid vector that contains an shRNA sequence targeting TurboGFP. The TurboGFP shRNA Control Vector is useful as a positive knockdown control in experiments using the MISSION TurboGFP positive control vector or in cell lines expressing TurboGFP. TurboGFP is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata.
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The TurboGFP shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The TurboGFP shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Application
MISSION® pLKO.1-puro TurboGFP™ shRNA control plasmid DNA has been used as lentiviral transduction and RNA interference assay.
To see more application data, protocols, vector maps visit sigma.com/shrna.
Informations légales
Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TurboGFP is a trademark of Evrogen Co.
En option
Réf. du produit
Description
Tarif
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Ze-Qiang Ren et al.
Pathology oncology research : POR, 26(2), 1153-1163 (2019-06-09)
Cullin-1 (CUL1) is an important factor for tumor growth and a potential therapeutic target for breast cancer therapy, but the molecular mechanism in triple-negative breast cancer (TNBC) is unknown. In the present study, CUL1 shRNA was transfected into BT549 and
The MEK2-binding tumor suppressor hDlg is recruited by E-cadherin to the midbody ring
Gaudet S, et al.
BMC Cell Biology, 12(1), 55-55 (2011)
Lorraine Springuel et al.
Cellular and molecular life sciences : CMLS, 73(24), 4739-4748 (2016-07-21)
Genomic instability drives cancer progression by promoting genetic abnormalities that allow for the multi-step clonal selection of cells with growth advantages. We previously reported that the IL-9-dependent TS1 cell line sequentially acquired activating substitutions in JAK1 and JAK3 upon successive
SMCHD1 regulates a limited set of gene clusters on autosomal chromosomes
Mason A G, et al.
Skeletal Muscle, 7(1), 12-12 (2017)
Anne Berberich et al.
Frontiers in oncology, 10, 747-747 (2020-06-26)
Background: Glioma therapy is challenged by the diffuse and invasive growth of glioma. Lysosomal protein transmembrane 5 (LAPTM5) was identified as an invasion inhibitor by an in vivo screen for invasion-associated genes. The aim of this study was to decipher
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