SAB4200733

Anti-IL6 high affinity antibody, Mouse monoclonal

clone IL6-4G1, purified from hybridoma cell culture

• Synonyme(s) : Anti-B-cell stimulatory factor 2 (BSF-2), Anti-CTL differentiation factor (CDF), Anti-Hybridoma growth factor, Anti-Interferon beta-2 (IFN-beta-2), Anti-Interleukin-6
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41
• Clone: IL6-4G1, monoclonal
• application: ELISA; IP
• Espèces réactives: human
• Technique(s): ELISA: suitable; immunoblotting: 2-4 μg/mL using concentrated supernatant of human osteosarcoma MG-63 cells treated with IL1β; immunoprecipitation (IP): 1-2 μg/test using concentrated supernatant of human osteosarcoma MG-63 cells treated with IL1β
• citations: 3

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Forme d'anticorps: purified from hybridoma cell culture
• Type de produit anticorps: primary antibodies
• Clone: IL6-4G1, monoclonal
• Forme: buffered aqueous solution
• Espèces réactives: human
• Concentration: ~1.0 mg/mL
• Technique(s): ELISA: suitable; immunoblotting: 2-4 μg/mL using concentrated supernatant of human osteosarcoma MG-63 cells treated with IL1β; immunoprecipitation (IP): 1-2 μg/test using concentrated supernatant of human osteosarcoma MG-63 cells treated with IL1β
• Isotype: IgG1
• Numéro d'accès UniProt: P05231
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... IL6(3569)

Description

Description générale

Anti-IL6 high-affinity antibody, mouse monoclonal (mouse IgG1 isotype) is derived from the IL6-4G1 hybridoma, produced by the fusion of mouse NS1 myeloma cells and splenocytes from a BALB/c mouse. Interleukin-6 (IL6) is mapped on the human chromosome at 7p15.3.

Spécificité

Monoclonal Anti-IL6 specifically recognizes human IL6 (KD ~4 x 10-11 M as measured by the Biacore system).

Immunogène

recombinant human IL6

Application

Anti-IL6 high-affinity antibody, mouse monoclonal may be used in:

• immunoblotting
• immunoprecipitation
• enzyme-linked immunosorbent assay (ELISA)

Actions biochimiques/physiologiques

Interleukin-6 (IL6) is a multifunctional cytokine that plays a wide variety of biological roles in numerous systems including the immune, nervous and endocrine systems. It is considered to perform as a primary regulator of acute and chronic inflammatory diseases. IL6 is an excellent target for clinical treatment against autoimmune disorders such as rheumatoid arthritis, juvenile idiopathic arthritis and Castleman′s disease. It is overexpressed in the pathogenesis of myeloma patients. IL6 might have elevated serum IL6 levels in patients with different kinds of malignant disease. IL6 at higher levels are also clinically implemented as biomarkers for acute coronary syndrome (ACS) , unstable angina, cardiovascular disease, heart attack and stroke.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable