SAB4200008

Anti-DVL3 (N-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-Dishevelled-3
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~95 kDa
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• Concentration: ~1.5 mg/mL
• Technique(s): immunoprecipitation (IP): 2.5-5 μg using HEK-293T cell lysate overexpressing human DVL3; western blot: 0.25-0.5 μg/mL using HEK-293T cell lysates overexpressing human DVL3
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... DVL3(1857)

Description

Description générale

Dishevelled 3 (DVL3) is a phosphoprotein rich in serine and threonine. It is widely expressed in fetal tissues and specifically expressed in various adult tissues. DVL3 gene is mapped to human chromosome 3q27.1

Spécificité

Anti-DVL3 (N-terminal) specifically recognizes human DVL3.

Application

Anti-DVL3 (N-terminal) antibody produced in rabbit has been used in immunoblotting and immunoprecipitation.

Actions biochimiques/physiologiques

Dishevelled 3 (DVL3) participates in the wingless-related integration site (Wnt) signaling pathway. It plays a key role in hippocampal neurogenesis regulation. Knockdown of DVL3 has been shown to attenuate the activation of the wnt signaling pathway by Wnt3A. DVL3 functions in redundant pathways with DVL1 and DVL2, and is necessary in normal cardiac, cochlea, and neural tube development in mice. DVL3, like other members of the DVL family, functions upstream of glycogen synthase kinase-3 (GSK3) to modulate the canonical wnt pathway. A frame shift mutation in DVL3 gene is implicated in Robinow syndrome. Allelic variation in the DVL3 gene may be associated with major depressive disorder.

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable