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PROTSIL1

Sigma-Aldrich

ProteoSilver™ Silver Stain Kit

Synonyme(s) :

Silver Staining Kit

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1 KIT
408,00 $

408,00 $

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1 KIT
408,00 $

About This Item

Code UNSPSC :
41105322
Nomenclature NACRES :
NA.32

408,00 $

En stockDétails

Utilisation

1 kit sufficient for 25 mini-gels (10 × 10 cm)

Niveau de qualité

200

Technique(s)

protein staining: suitable

Catégories apparentées

Description générale

Conveniently packaged, extremely stable, and highly sensitive, ProteoSilver is an ideal product for any protein scientist. The kit contains prepared solutions of silver staining reagents along with detailed instructions to achieve optimal results. With a detection limit of 0.1 ng/mm2 of protein (BSA) and an extremely low background, ProteoSilver leads to superior detection of low abundance proteins.

Application

ProteoSilver™ Silver Stain Kit has been used for staining of the gels for visualizing proteins.[1]

Caractéristiques et avantages

  • Premixed and preweighed solutions reduce time and cost of purchasing and preparing individual components
  • Optimized protocols help establish conditions for best results
  • High sensitivity and low background ensure very low abundance proteins can be detected and resolved from other proteins
  • MALDI Compatible - Protein spots of interest can be further characterized by mass spectrometry
  • Room Temperature Stability allows easy and convenient storage

Informations légales

ProteoSilver is a trademark of Sigma-Aldrich Co. LLC

Remplacé(e)(s) par

Réf. du produit
Description
Tarif

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Muta. 2 - Repr. 1B - Skin Corr. 1A - Skin Sens. 1 - STOT SE 2 - STOT SE 3

Organes cibles

Eyes,Central nervous system, Respiratory system

Code de la classe de stockage

3 - Flammable liquids

Point d'éclair (°F)

132.8 °F - closed cup

Point d'éclair (°C)

56 °C - closed cup

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
0000368055
0000368055
SLCR2401
SLCR2403
SLCR2402

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Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Jean-Paul Lasserre et al.
Electrophoresis, 27(16), 3306-3321 (2006-07-22)
Study of the complexome - all the protein complexes of the cell - is essential for a better understanding and more global vision of cell function. Using two-dimensional blue native/SDS-PAGE (2-D BN/SDS-PAGE) technology, the cytosolic and membrane protein complexes of
Yufan Zhang et al.
Materials today. Bio, 15, 100317-100317 (2022-06-28)
Polymer dots (Pdots) have been applied to imaging lymph nodes (LNs) and lymphatic vessels (LVs) in living mice and rats. However, the mechanism of absorption, distribution, metabolism, and excretion of Pdots in LNs and LVs is still unclear. Therefore, the
Andrea Miccoli et al.
International journal of molecular sciences, 22(7) (2021-04-04)
In jawed vertebrates, adaptive immune responses are enabled by T cells. Two lineages were characterized based on their T cell receptor (TcR) heterodimers, namely αβ or γδ peptide chains, which display an Ig domain-type sequence that is somatically rearranged. γδ
Yong-Feng Han et al.
The Plant cell, 28(5), 1215-1229 (2016-04-27)
The mechanism by which MORPHEUS' MOLECULE1 (MOM1) contributes to transcriptional gene silencing has remained elusive since the gene was first identified and characterized. Here, we report that two Arabidopsis thaliana PIAS (PROTEIN INHIBITOR OF ACTIVATED STAT)-type SUMO E3 ligase-like proteins
Jing Tian et al.
eLife, 7 (2018-06-29)
In obesity, elevated insulin causes fatty liver by activating the gene encoding SREBP-1c, a transcription factor that enhances fatty acid synthesis. Two transcription factors, LXRα and C/EBPβ, are necessary but not sufficient for insulin induction of hepatic SREBP-1c mRNA. Here
Afficher tous les articles scientifiques apparentés

Articles

Protein Staining Reagents Selection Guide

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

Sample Prep & Gel Electrophoresis Troubleshooting

Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.

Bis Tris Polyacrylamide Gel Electrophoresis Technology

Bis-Tris SDS-PAGE gel casting kits with mPAGE® TurboMix technology for rapid, reliable gel casting and protein separation.

Protocoles

Lyse cellulaire et extraction protéique pour le western blotting

Protocole de préparation d'échantillons pour la lyse cellulaire et une bonne extraction des protéines à partir de cellules et de tissus en culture en vue d'un western blotting ultérieur.

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

Coulage manuel de gels de polyacrylamide pour PAGE et SDS-PAGE à l'aide des kits de coulage de gels Bis-Tris mPAGE® TurboMix

Vue d'ensemble des propriétés chimiques des gels de polyacrylamide et détail des consignes à suivre pour les couler à l'aide des kits de coulage de gels Bis-Tri mPAGE® TurboMix.

Hand-casting gels for PAGE and SDS-PAGE using mPAGE® TurboMix Bis-Tris Gel Casting Kits

Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

Questions

1–3 of 3 Questions  

  1. Quality release date

    1 answer

    1. The quality release date can be found on the lot-specific Certificate of Analysis. Please see the link below to access the appropriate lot Certificate:
      https://www.sigmaaldrich.com/product/sigma/protsil1#product-documentation

      Helpful?

  2. What the expired date of this product 

    1 answer

    1. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

  3. Can  this silver stain kit stain proteins without cysteines?

    1 answer

    1. This kit has not been tested for use in samples with specifically no cysteine. There is a modification in the protocol should the sample have few cysteine residues. While cysteine is important in the mechanism of silver staining, the link below indicates that it may be possible:
      https://pubmed.ncbi.nlm.nih.gov/2426985/#:~:text=Proteins%20without%20cysteine%20stained%20negatively,they%20contained%20covalently%20attached%20lipids.

      See the link below to the product technical bulletin:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/156/682/protsil1bul.pdf

      Helpful?

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