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MAK567

Sigma-Aldrich

Pyruvate Dehydrogenase Activity Assay Kit

sufficient for 100 colorimetric tests

Synonyme(s) :

PDH Activity Assay Kit

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About This Item

Code UNSPSC :
41116104

Utilisation

sufficient for 100 colorimetric tests

Niveau de qualité

100

Méthode de détection

colorimetric

Température de stockage

−20°C

Informations sur le gène

human ... PDHA1(5160) , PDHA2(5161 ) , PDHB(5162) , PDHX(8050)
mouse ... PDHA1(18597) , PDHA2(18598) , PDHB(68263) , PDHX(27402)
rat ... PDHA1(29554) , PDHA2(117098) , PDHB(289950) , PDHX(311254)

Description générale

Pyruvate Dehydrogenase (PDH) is a mitochondrial enzyme which has an important role in carbohydrate metabolism. Combining with dihydrolipoyl transacetylase (E2) and dihydrolipoyl dehydrogenase (E3), PDH (E1) forms a well-characterized enzyme complex (PDC). In the presence of NAD+ and CoA, PDH catalyzes the conversion of pyruvate into Acetyl-CoA and CO2. Thus, PDH links the glycolysis metabolic pathway (cytoplasm) to the citric acid cycle (mitochondria). The Pyruvate Dehydrogenase Activity Assay Kit provides a sensitive, simple, fast, direct and convenient way for the detection of PDH activity in various samples, based on a colorimetric method. In the assay, PDH activity changes a colorless developer to a colored product. The amount of the colorimetric (450 nm) product is proportional to the enzymatic activity present in the sample. The kit can detect PDH activity that is lower than 0.1 mU, in a variety of samples.

Application

Pyruvate Dehydrogenase (PDH) Activity Assay Kit has been used in the colorimetric measurement of PDH Activity in biological samples.

Caractéristiques et avantages

Sensitive, simple, fast, direct and convenient way for the detection of PDH activity in various biological samples.
Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.

Click here to download a calculator excel file

Adéquation

Suitable for measuring PDH Activity in adherent or suspension cells, from an animal or plant source.

Définition de l'unité

One unit of pyruvate dehydrogenase is the amount of enzyme that will generate 1.0 µmole of NADH per minute at 37 °C at pH=7.5.

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H315,H319,H334,H341,H351

Classification des risques

Carc. 2 - Eye Irrit. 2 - Muta. 2 - Resp. Sens. 1 - Skin Irrit. 2

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Rapid intramolecular coupling of active sites in the pyruvate dehydrogenase complex of Escherichia coli: mechanism for rate enhancement in a multimeric structure
Danson M J, et al.
Proceedings of the National Academy of Sciences of the USA, 75(11), 5386-5390 (1978)
Self-assembly and catalytic activity of the pyruvate dehydrogenase multienzyme complex from Bacillus stearothermophilus
Domingo G J, et al.
European Journal of Biochemistry, 266(3), 1136-1146 (1999)
Structural basis for inactivation of the human pyruvate dehydrogenase complex by phosphorylation: role of disordered phosphorylation loops
Kato M, et al.
Structure (Oxford, United Kingdom), 16(12), 1849-1859 (2008)
An improved spectrophotometric assay of pyruvate dehydrogenase in lactate dehydrogenase contaminated mitochondrial preparations from human skeletal muscle
Chretien D, et al.
Clinica Chimica Acta; International Journal of Clinical Chemistry, 240(2), 129-136 (1995)
A key role for mitochondrial gatekeeper pyruvate dehydrogenase in oncogene-induced senescence
Kaplon J, et al.
Nature, 498(7452), 109-112 (2013)
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