It is recommended to use a dounce homogenizer on ice or perform mild sonication on ice to lyse the cells. The assay buffer contains detergent but will not lyse the cells by simply incubating in it.
MAK082
Aldehyde Dehydrogenase Activity Colorimetric Assay Kit
sufficient for 100 colorimetric tests
Synonyme(s) :
ALDH Activity Assay Kit
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84
Produits recommandés
Utilisation
sufficient for 100 colorimetric tests
Méthode de détection
colorimetric
Maladie(s) pertinente(s)
cardiovascular diseases; cancer; hematological disorder
Température de stockage
−20°C
Description générale
Aldehyde dehydrogenases (ALDH) are a family of enzymes that catalyze the metabolism of exogenous and endogenous aldehydes, preventing the accumulation of potentially reactive and toxic aldehydes and their metabolites. In addition to their role in aldehyde metabolism, ALDH enzymes also play critical roles in other cellular processes such as cell proliferation, differentiation, and survival.[1][2] Mutations in ALDH family members have been linked to numerous diseases such as heart disease, cataracts, and certain cancers. ALDH activity may also serve as a marker for certain stem cell populations including hematopoietic stem cells and certain cancer stem cells.[3]
Application
Caractéristiques et avantages
Compatible with high-throughput handling systems.
Adéquation
Suitable for the measurement of aldehyde dehydrogenase (ALDH) in a variety of samples including tissue and cells
Principe
The Aldehyde Dehydrogenase Activity Colorimetric Assay kit provides a simple and direct procedure for measuring NAD-dependent ALDH activity in a variety of samples. ALDH activity is determined by a coupled enzyme assay in which acetaldehyde is oxidized by ALDH generating NADH, which reacts with a probe generating a colorimetric (450 nm) product proportional to the ALDH activity present. One unit of ALDH is the amount of enzyme that will generate 1.0 μmole of NADH per minute at pH 8.0 at room temperature.
Mention d'avertissement
Danger
Mentions de danger
Classification des risques
Carc. 1B - Eye Dam. 1 - Skin Corr. 1B
Code de la classe de stockage
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Faites votre choix parmi les versions les plus récentes :
Certificats d'analyse (COA)
Lot/Batch Number
Vous ne trouvez pas la bonne version ?
Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Xiaoman Li et al.
Experimental hematology & oncology, 11(1), 2-2 (2022-01-22)
Tanshinone II A is an effective component extracted from Salvia miltiorrhiza and the roles of Tanshinone IIA in regulating the stemness of tumor cells remain unclear. This work aims to explore the roles and underlying mechanisms of Tanshinone IIA in
Yilu Qin et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 8(2), 2002232-2002232 (2021-01-30)
Cancer stem cells (CSCs) are considered the roots of cancer metastasis and recurrence (CSCs), due in part to their self-renewal and therapy resistance properties. However, the underlying mechanisms for the regulation of CSC stemness are poorly understood. Recently, increasing evidence
Characterization of the CD49f+/CD44+/CD24- single-cell derived stem cell population in basal-like DCIS cells.
Duru N, et al.
Oncotarget, 7(30), 47511-47511 (2016)
A Novel Long Non-Coding RNA lnc030 Maintains Breast Cancer Stem Cell Stemness by Stabilizing SQLE mRNA and Increasing Cholesterol Synthesis.
Yilu Qin et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 9(24), e2204046-e2204046 (2022-08-26)
Ting Liu et al.
Bioengineered, 12(1), 7143-7155 (2021-09-22)
Cancer stem cell (CSC) has been confirmed to trigger tumor occurrence and progression and CSC can develop strategies to maintain a lower reactive oxygen species (ROS) level compared to cancer cells. However, the mechanisms contributing to ROS homeostasis in CSC
Articles
AldeRed™ 588-A detects ALDH activity in cancer stem cells, aiding in cancer research.
-
Is it possible for the assay buffer to lyse the cells without the use of additional instrumentation such as a sonicator?
1 answer-
Helpful?
-
-
Is there a general range expected for the activity of the ALDH positive control? Given variances in room temperature, etc.
1 answer-
The positive control does not have an expected activity range.
Helpful?
-
-
What is the kit Sensitivity? thanks,
1 answer-
This assay kit can detect < 0.5 mU/well of ALDH activity (based on the unit definition) in a variety of samples. Unit Definition: One unit is the amount of enzyme that will generate 1.0 umol of NADH per min at pH 8 at room temperature. This is a sensitive assay for measuring ALDH activity in various biological samples and the kit is also suitable for high throughput analyses.
Helpful?
-
Active Filters
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique
