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Key Documents

MAK074

Sigma-Aldrich

Ascorbic Acid Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonyme(s) :

Vitamin C Assay Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 100 colorimetric or fluorometric tests

Méthode de détection

colorimetric
fluorometric

Température de stockage

−20°C

Description générale

The new Ascorbic Acid Assay Kit, MAK505, is now available! Ascorbic Acid, also known as Vitamin C, is a six-carbon lactone produced by plants and some animal species but not by humans and other primates. Ascorbic acid functions as an enzymatic cofactor for multiple enzymes, serving as an electron donor for monooxygenases and dioxygenases. Ascorbic acid also functions as a powerful antioxidant, particularly in regards to reactive oxygen species.

Adéquation

Suitable for use with biological fluids, food samples, and growth media. Proteins present in the sample can interfere with this assay. Protein containing samples are best analyzed using the Ascorbic Acid Assay II (MAK075). Alternatively, samples can be deproteinized using a 10 kDa Molecular Weight Cut-Off Spin Filter.

Principe

In this assay, Ascorbic Acid concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/ λem = 587 nm) product, proportional to the ascorbic acid present.

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Réf. du produit
Description
Tarif

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Health hazard

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Eye Irrit. 2 - Resp. Sens. 1 - Skin Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

188.6 °F - closed cup

Point d'éclair (°C)

87 °C - closed cup


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