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M7383

Sigma-Aldrich

Malic Dehydrogenase from porcine heart

≥400 units/mg protein (biuret), ammonium sulfate suspension

Synonyme(s) :

L-Malate: NAD+ oxidoreductase, MDH, Malate Dehydrogenase

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About This Item

Numéro de classification (Commission des enzymes):
1.1.1.37 (BRENDA, IUBMB)
Numéro CE :
232-622-5
Numéro MDL:
MFCD00131567
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Forme

ammonium sulfate suspension

Activité spécifique

≥400 units/mg protein (biuret)

Activité étrangère

Glutamic-Oxalacetic Transaminase ≤0.01%
Glutamic-Pyruvic Transaminase ≤0.01%

Température de stockage

2-8°C

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Description générale

Cytoplasmic Isozyme

Application

Malic dehydrogenase has been used in a study to assess the use of a long-wavelength homogeneous enzyme immunoassay for the determination of amikacin in water samples. It has also been used in a study to investigate the lipid metabolism in a liver exposed to cadmium.

Actions biochimiques/physiologiques

The surfactant cetyl (hexadecyl) trimethylammonium bromide inhibits the cytoplasmic isoform of porcine heart malate dehydrogenase but has no effect on the mitochondrial form. SDS however; inhibits both isoforms.

Définition de l'unité

One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25 °C.

Forme physique

Suspension in 3.2 M (NH4)2SO4, 0.1 M KH2PO4, pH 7.0

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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Glutamic-Oxalacetic Transaminase from porcine heart Type I, ammonium sulfate suspension, 200-500 units/mg protein

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G2751

Glutamic-Oxalacetic Transaminase from porcine heart

Citrate Synthase from porcine heart ammonium sulfate suspension, ≥100 units/mg protein

Sigma-Aldrich

C3260

Citrate Synthase from porcine heart

P Benoist et al.
Analytical biochemistry, 187(2), 337-344 (1990-06-01)
Nanogram amounts of soluble aminopeptidases, dehydrogenases, and esterases were detected by nondenaturing ultralow gelling point agarose-polyacrylamide gel electrophoresis (ULGA-PAGE). Cytosolic fractions from Frankia sp. were electrophoresed at 4 degrees C in the presence of Co2+, Zn2+, or Mg2+ ions. Then
K Smith et al.
Biochimica et biophysica acta, 884(1), 109-118 (1986-10-29)
The cationic surfactant, cetyl (hexadecyl) trimethylammonium bromide (CTAB), completely inactivates porcine heart cytoplasmic malate dehydrogenase (L-malate:NAD+ oxidoreductase, EC 1.1.1.37) at concentrations (of surfactant) which do not affect the activity of the mitochondrial isoenzyme. These concentrations are close to, or higher
Marie Knockaert et al.
The Journal of biological chemistry, 277(28), 25493-25501 (2002-04-20)
Numerous inhibitors of cyclin-dependent kinases and glycogen synthase kinase-3 (GSK-3) are being developed in view of their potential applications against cancers and neurodegenerative disorders. Among these, paullones constitute a family of potent and apparently selective cyclin-dependent kinase and GSK-3 inhibitors.
Ethel V Larregle et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 46(5), 1786-1792 (2008-03-11)
We investigated the effect of exposition to cadmium (Cd, 15ppm for 8 weeks) through drinking water on liver lipid metabolism in adult male Wistar rats. As compared to metal non-exposed (control) rats, the serum triglycerides, cholesterol and LDL+VLDL cholesterol concentrations
M L Sánchez-Martínez et al.
Talanta, 78(1), 305-309 (2009-01-29)
A simple and rapid homogeneous enzyme immunoassay involving the use of the malic dehydrogenase enzyme and a long-wavelength fluorophor, the oxazine Cresyl Violet, is proposed for the determination of the antibiotic amikacin in water samples. An enzymatic tracer has been
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