L1378

L-Lactic Dehydrogenase from bovine muscle

Type X, ammonium sulfate suspension, ≥600 units/mg protein

• Synonyme(s) : (S)-Lactate: NAD⁺ oxidoreductase, L-LDH, LAD, LD, Lactate
• Numéro CAS: 9001-60-9
• Numéro de classification (Commission des enzymes): 1.1.1.27 (BRENDA, IUBMB)
• Numéro CE : 232-617-8
• Numéro MDL: MFCD00131466
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: bovine muscle
• Type: Type X
• Pureté: 3.0—25.0 mg protein/mL (biuret)
• Forme: ammonium sulfate suspension
• Activité spécifique: ≥600 units/mg protein
• Fabricant/nom de marque: Sigma-Aldrich
• Technique(s): activity assay: suitable
• Couleur: white
• pH: 7.5
• Numéro d'accès UniProt: P19858; Q5E9B1
• Application(s): life science and biopharma
• Activité étrangère: pyruvate kinase ≤0.01%
• Température de stockage: 2-8°C
• Informations sur le gène: cow ... LDHA(281274) , LDHB(281275)

Description

Description générale

Research Area: Cell Signaling
Lactate dehydrogenase (LDH) is a key enzyme in the anaerobic metabolic pathway and falls under the class of oxidoreductases. This enzyme is found in a wide range of organisms, including both plants and animals. It is widely distributed across all tissues. LDH is primarily a cytoplasmic enzyme, with particularly high concentrations in muscle, liver, and kidney tissues.

Application

L-Lactic Dehydrogenase from bovine muscle has been used in lactate assay to assess lactate tissue accumulation.

Actions biochimiques/physiologiques

Lactate dehydrogenase (LDH) plays a critical role as a checkpoint in gluconeogenesis and DNA metabolism. It plays a vital role in maintaining homeostasis during periods of low oxygen availability. As an oxidoreductase enzyme, lactate dehydrogenase catalyzes the reversible conversion of pyruvate to lactate using NADH. This enzyme is crucial for the anaerobic metabolism of glucose when oxygen is either absent or in short supply. LDH is the sole serum biomarker effective for evaluating metastatic melanomas.

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Définition de l'unité

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

Forme physique

Crystalline suspension in 2.4 M (NH₄)₂SO₄ solution, pH 6.0

Remarque sur l'analyse

Protein determined by biuret.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)