The activity of the product should be 25 IU/mL, based on the formulation and the IU of the raw materials used. The product has a specification of >= 0.9mg/mL, and the raw material has an activity specification of >= 25 IUM. Therefore, depending on the lot the customer received, if the assay of the lot they received was 1mg/mL, then the activity would be 25 IU/mL.
I2521
ITS+1 Liquid Media Supplement (100×)
ITS+1 Liquid Media Supplement (100×)
liquid, sterile-filtered, BioReagent, suitable for cell culture
Synonyme(s) :
Insulin-transferrin-sodium selenite, linoleic-BSA supplement
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5 ML
79,80 $
79,80 $
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Devis pour commande en gros
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Changer de vue
5 ML
79,80 $
About This Item
Code UNSPSC :
12352205
Nomenclature NACRES :
NA.75
79,80 $
Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
Devis pour commande en gros
Produits recommandés
Stérilité
sterile-filtered
Gamme de produits
BioReagent
Forme
liquid
Technique(s)
cell culture | mammalian: suitable
Impuretés
endotoxin, tested
Conditions d'expédition
ambient
Température de stockage
2-8°C
Description générale
Serum-free culture environment avoids the variability and contaminants that can be introduced by serum. Serum-free formulations that substitute a purified form of the factors normally supplied by serum are suitable for many in vitro growth and differentiation studies. These factors include insulin, transferrin, selenium, pyruvate, and ethanolamine. The addition of other components varies greatly, depending on the cell type being studied and the basal medium employed. ITS is a mixture of insulin, human transferrin (partially iron-saturated), and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g., MEM, RPMI-1640) and complex media (e.g., Ham′s F-12, DME/F-12, MEM) with sodium pyruvate.
Application
ITS+1 Liquid Media Supplement (100×) has been used as a supplement in high-glucose Dulbecco′s Modified Eagle Medium (DMEM) for differentiation of mouse mesenchymal stem cells (mMSCs) to chondrocytes. It has also been used as a supplement in basal differentiation medium for differentiation of human-induced pluripotent stem cell (hiPSC) into osteoblasts under serum-free conditions.
Composants
Contains 1.0 mg/ml bovine insulin, 0.55 mg/ml human transferrin (substantially iron-free), 0.5 μg/ml sodium selenite, 50 mg/ml bovine serum albumin and 470 μg/ml linoleic acid at the 100x concentration.
Notes préparatoires
Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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Could you provide information on the unit of activation for the ITS+1 liquid media supplement - product I2521? The media I want to use requires 0.023U/mL of insulin in 500mL of RPMI, and I need to understand the activation units for the insulin supplement. The product information only mentions a 1mg/mL concentration of insulin.
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