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H8038

Sigma-Aldrich

Anti-Histone Deacetylase 8 (HDAC8) antibody produced in rabbit

enhanced validation

affinity isolated antibody, buffered aqueous solution

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~43 kDa

Espèces réactives

human, mouse

Validation améliorée

recombinant expression
Learn more about Antibody Enhanced Validation

Technique(s)

immunocytochemistry: 1-2 μg/mL using cultured 293T cells expressing recombinant human HDAC8
immunoprecipitation (IP): 1-2 μg using extract of 293T cells expressing recombinant human HDAC8
western blot: 2-4 μg/mL using whole extract of human colon adenocarcinoma SW620 cells and a chemiluminescent detection reagent

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... HDAC8(55869)
mouse ... Hdac8(70315)

Catégories apparentées

Description générale

Histone deacetylases (HDACs) are competing enzymes, belonging to histone deacetylase family. There are two classes of HDACs with six to seven different types of HDACs proteins. HDAC1,HDAC2, HDAC3, and HDAC8 belong to Class I HDACs and HDAC4, HDAC6, HDAC7, HDAC9, and HDAC10 belong to Class II HDACs. Class I HDACs consists of a single deacetylase domain at the N-termini and diversified C-terminal regions, while Class II contains a deacetylase domain at C-terminal position. Studies show that HDAC8 is a sex-linked gene located on the chromosome at position Xq21.2 - q21.3. HDAC8 gene has a molecular weight of 43kDa and it encodes a 377 amino acid protein. It is present within the nucleas. HDAC8 mRNA is seen in heart, lung, kidney, and pancreas.
The antibody is affinity-purified using the immunizing peptide immobilized on agarose.

Immunogène

synthetic peptide corresponding to amino acid residues 352-370 of human HDAC8, conjugated to KLH. The corresponding sequence in mouse differs by one amino acid.

Application

Anti-Histone Deacetylase 8 (HDAC8) antibody produced in rabbit has been used in western blotting, immunohistofluorescence and immunoblotting.

Actions biochimiques/physiologiques

Histone deacetylation results in transcription repression leading to the formation of tight nucleosomal structure which prevents DNA accessing. {5} HDAC8 controls HDAC activity on H4 histone peptide substrates. HDAC8 is similar to the HDAC class I enzymes. In vitro expression and activity of HDAC8 was examined using FLAG tagged- HDAC8 and HDAC1. These were transfected into HeLa cells and Sf9 insect cells. After the immunoprecipitation of cell lysates the expression results were confirmend using Western blotting. Studies show that HDAC8 may play a role in role in transcriptional regulation and could possibly be regulated in a temporal or compartment-specific manner.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Expression of class I histone deacetylases in ipsilateral and contralateral hemispheres after the focal photothrombotic infarction in the mouse brain
Demyanenko S, et al.
Translational Stroke Research, 9(5), 471-483 (2018)
Redox signaling, alkylation (carbonylation) of conserved cysteines inactivates class I histone deacetylases 1, 2, and 3 and antagonizes their transcriptional repressor function
Doyle K and Fitzpatrick FA
Test, 285(23), 17417-17424 (2010)
Marisela Agudelo et al.
PloS one, 11(6), e0156421-e0156421 (2016-06-02)
Epigenetic mechanisms have been shown to play a role in alcohol use disorders (AUDs) and may prove to be valuable therapeutic targets. However, the involvement of histone deacetylases (HDACs) on alcohol-induced oxidative stress of human primary monocyte-derived dendritic cells (MDDCs)
Profile of class I histone deacetylases (HDAC) by human dendritic cells after alcohol consumption and in vitro alcohol treatment and their implication in oxidative stress: role of HDAC inhibitors trichostatin A and mocetinostat
Agudelo M, et al.
Testing, 11(6), e0156421-e0156421 (2016)
J J Buggy et al.
The Biochemical journal, 350 Pt 1, 199-205 (2000-08-06)
Histone deacetylases (HDACs) are a growing family of enzymes implicated in transcriptional regulation by affecting the acetylation state of core histones in the nucleus of cells. HDACs are known to have key roles in the regulation of cell proliferation [Brehm

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