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Principaux documents

GE17-0507-01

Sigma-Aldrich

Mono S® HR 16/10

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About This Item

Code UNSPSC :
41115700
Nomenclature NACRES :
SB.52

ligand

-OCH2CH(OH)CH2OCH2CH(OH)CH2SO3-

Matériaux

PEEK column

Description

Ion Exchanger Type (Methyl sulfonate strong cation)

Conditionnement

pkg of 1 ea

Paramètres

435 psi

Taille du lit

16 mm × 100 mm

Volume du lit

20 mL

D.I. de la colonne

16 mm

Taille

Matrice

polymeric

Groupe de la matrice active

methyl sulfonate phase

Taille moy. des particules

10 μm

cleaning

1-14

Plage de travail

2-12

Technique de séparation

cation exchange

Description générale

Mono S® HR 16/10 is a strong cation exchanger prepacked with MonoBeads. The column is an excellent choice for the polishing step in purification of proteins, peptides, and other biomolecules when high purity is required. The 20 mL bed volume of the column offer high capacity.

Application

  • Monodisperse 10 μm porous beads yield high resolution, dynamic capacity, reproducibility, and durability.
  • Maximum resolution is achieved from the efficiency of small, perfectly spherical monodispersed particles optimally packed in columns coupled with the excellent selectivities of the Q ion exchangers.
  • Compatible with ÄKTA® design, FPLC™ System, and other high-performance LC systems.
  • MonoBeads are also available as Mono P® for chromatofocusing

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Autres remarques

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

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Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Informations légales

FPLC is a trademark of Cytiva
Mono P is a registered trademark of Cytiva
Mono S is a registered trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

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Certificats d'analyse (COA)

Lot/Batch Number

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Articles

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protocoles

MonoBeads: Purification of Milligram Quantities with Highest Resolution

This page covers using Monobeads for purification of proteins, peptides, or oligonucleotides.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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