F5392

Fibroblast Growth Factor-Basic from bovine pituitary

~90% (SDS-PAGE and N-terminal analysis), lyophilized powder, suitable for cell culture

• Synonyme(s) : FGF2, bFGF
• Numéro CAS: 106096-93-9
• Numéro MDL: MFCD00081382
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Nom du produit: Fibroblast Growth Factor-Basic from bovine pituitary, FGF-Basic, suitable for cell culture
• Source biologique: bovine pituitary glands
• Niveau de qualité: 200
• Essai: ~90% (SDS-PAGE and N-terminal analysis)
• Forme: lyophilized powder
• Puissance: 0.03-3.0 ng/mL ED₅₀/EC₅₀
• Qualité: endotoxin tested
• Poids mol.: 16.4 kDa
• Conditionnement: pkg of 1 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Technique(s): cell culture | mammalian: suitable
• Impuretés: <0.5 EU/vial
• Couleur: white
• Numéro d'accès UniProt: P03969
• Température de stockage: −20°C
• Informations sur le gène: bovine ... FGF2(281161)

Description

Description générale

The fibroblast growth factor-2 (FGF2) gene is predominantly expressed in uterus, cornea and vascular endothelial cells.[1] The FGF2 gene is mapped to cattle chromosome 17.[2]

Application

A natural mitogenic growth factor used in cell culture applications to study cell signaling and to promote fibroblast and endothelial cell growth and survival.
Fibroblast Growth Factor-Basic from bovine pituitary has been used:

• In animal and human cell culture[3]
• In neuronal induction of adipose and bone marrow-derived rat mesenchymal stem cells[4]
• In the generation of directly reprogramming fibroblast[5]
• In human glioma cell culture[6]

Actions biochimiques/physiologiques

Fibroblast growth factor-2 (FGF2) stimulates cell proliferation, differentiation and migration. It is also known to participate in angiogenesis and carcinogenesis. During the estrous cycle, FGF2 expression is found to be maximum in porcine conceptuses and endometrium. Upregulation of this gene is also observed in pregnancy during peri-implantation. FGF2 is involved in embryogenesis, tissue repair and other metabolic pathways.[1]

Forme physique

Lyophilized from a 0.2 μm filtered solution in sodium phosphate and sodium chloride containing 0.1 mg bovine serum albumin

Notes préparatoires

Purified glands by a modification of the method of Gospodarowicz, et al.[7]

Remarque sur l'analyse

The proliferative activity is tested in culture using fetal bovine heart endothelial cells.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)