E8656
Heat-Labile Enterotoxin, B subunit from E. coli
recombinant, expressed in Pichia pastoris, >90% (SDS-PAGE), lyophilized powder
Synonyme(s) :
LTB
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About This Item
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.77
Produits recommandés
Produit recombinant
expressed in Pichia pastoris
Niveau de qualité
Pureté
>90% (SDS-PAGE)
Forme
lyophilized powder
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Application
Heat-Labile Enterotoxin, B subunit (LTB) from E. coli has been used in enzyme-linked immunosorbent assay (ELISA).
Actions biochimiques/physiologiques
Enterotoxigenic Escherichia coli causes diarrhea through its heat-labile enterotoxin (LT). The LT is a periplasmic protein composed of one A subunit (LTA, 27 kDa) and five non-covalently associated B subunits (LTB, 11.6 kDa each) forming a ring-like pentamer. LTB has high affinity towards the toxin receptor ganglioside GM1, a glycosphingolipid found ubiquitously on the surface of mammalian cells. Ganglioside GM1 facilitates the delivery of the A subunit to the cytosol of the target cell resulting in persistent synthesis of cAMP and subsequently diarrhea. This characteristic makes LTB a good label for microglial cells (due to the enrichment of ganglioside GM1 on their cell surface). In addition, studies made mostly with animal models demonstrated that recombinant LTB could stimulate strong serum and mucosal immune responses against LT. Other studies have indicated that LTB could be used as a potent mucosal adjuvant.
Caractéristiques et avantages
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Forme physique
Lyophilized from a solution containing Heat-Labile Enterotoxin, B subunit in 0.05 M Tris buffer, pH 7.5, 0.2 M NaCl, 3 mM NaN3, and 1 mM sodium EDTA.
Remarque sur l'analyse
The activity is measured by ELISA using ganglioside GM1-coated plates (Cat. No. G7641). LTB at various concentrations is incubated on the ganglioside GM1 coated wells, followed by anti-cholera toxin antibody (Cat. No. C3062), and peroxidase-labeled goat anti-rabbit IgG (Cat.No. A0545) as the secondary antibody. Binding saturation of 50% may be achieved with = 0.12 μg/mL of LTB.
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Acute Tox. 4 Dermal - Aquatic Chronic 3
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
Certificats d'analyse (COA)
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