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DMN70

Sigma-Aldrich

GenElute™ Direct mRNA Miniprep Kits

sufficient for 70 purifications

Synonyme(s) :

GenElute™ Direct mRNA Miniprep Kit, GenElute™ mRNA Kit, Gen Elute

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About This Item

Code UNSPSC :
41105501
Nomenclature NACRES :
NA.52

Utilisation

sufficient for 70 purifications

Température de stockage

15-25°C

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Description générale

The GenElute™ Direct mRNA Miniprep kit provides a convenient format to isolate polyadenylated mRNA directly from mammalian cells and tissues. The direct mRNA isolation procedure is based on that of Badley. Up to 107 mammalian cells or 40 mg tissue are lysed and homogenized, either with the filtration columns provided or with
a mechanical homogenizer. RNase is eliminated during a 10 minute proteinase K digestion. Sodium chloride is added, and polyadenylated RNA is captured on oligo(dT) polystyrene beads during a 10 minute incubation. For further enrichment, RNA may be released from the beads into fresh lysis solution and recaptured with the original beads. After 3 washes in a spin column, purified mRNA is eluted in 100 μl of 10 mM Tris-HCl, pH 7.4.

Application

GenElute™ Direct mRNA Miniprep Kit has been used to isolate mRNA from various tissues and cells.
The GenElute Direct Kit mRNA kit provides convenient procedures for isolating polyadenylated mRNA from previously prepared total RNA or directly from mammalian cells and tissues.

Caractéristiques et avantages

  • mRNA captured on oligo(dT) polystyrene beads in 10 minutes, with no mixing or rocking (Fig. 1)
  • Poly (A)+ mRNA isolated from total RNA in 40 minutes (Fig. 2) or 60 minutes directly from cells and tissues (Fig. 3)
  • Oligo(dT) polystyrene beads require fewer wash steps

Autres remarques

Use for isolating mRNA directly from mammalian cells or tissues.
For additional information, please see www.sigma-aldrich.com/mrna.

Informations légales

GenElute is a trademark of Sigma-Aldrich Co. LLC

Composants de kit seuls

Réf. du produit
Description
  • Collection tube 70 ea
  • Elution solution 10 mL
  • Filtration columns with tubes 70 ea
  • Lysis solution 120 mL
  • 5 M NaCl 8 mL
  • Oligo(dT)-polystyrene beads 2 mL
  • Proteinase K 25 mg
  • Spin columns with tubes 70 ea
  • 40% Glycerol solution 3 mL
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Pictogrammes

Health hazardCorrosionExclamation mark
GHS08,GHS05,GHS07

Mention d'avertissement

Danger

Mentions de danger

H290,H315,H319,H334,H335

Classification des risques

Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Identification and charactrization of glucoamylase from the fungus Thermomyces lanuginosus
Thor S Thorsen
Biochimica et Biophysica Acta (2006)
Human embryonic stem cell differentiation on tissue engineering scaffolds: effects of NGF and retinoic acid induction.
Inanc B
Tissue Engineering: Part A, 14(6), 955-964 (2008)

Articles

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