DAB (3,3′-Diaminobenzidine Tetrahydrochloride) Liquid Substrate Dropper System has been developed for use in immunohistology and immunoblotting procedures as a precipitating substrate for the detection of peroxidase activity. DAB is the immunohistology substrate of choice because it produces an intense brown stain that is easily observed. The end product is resistant to alcohol, therefore, a variety of counterstains and mounting media can be used with the DAB Liquid Substrate Dropper System. DAB Liquid Substrate Dropper System provides all of the chromogen and buffer/peroxide solutions needed to produce a fast and convenient DAB substrate solution.
Application
3,3′-Diaminobenzidine (DAB) Liquid Substrate Dropper System has been used as a developer in immunohistogical examination.[1][2][3]
Attention
Stable at least one year at 2-8 °C.
Quantité
Each DAB liquid substrate dropper system will provide ten individual application dropper bottles of 10 mL of a fast and convenient DAB substrate solution.
Composants de kit seuls
Réf. du produit
Description
10× DAB Liquid Chromogen 10 mL
premeasured, ready-to-use buffer/peroxide solution for individual application 10 x 9
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
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The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 30(10), 1079-1082 (1982-10-01)
Three metallic ions, NiCl2, CoCl2, and CuSO4, were found to modify the color of the normally brown diaminobenzidine (DAB) reaction. The colors ranged from purplish blue (NiCl2), dark blue/bluish black (CoCl2), to greyish blue (CuSO4). We have found that the
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