BHL-89

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Code UNSPSC : 41106514

Propriétés

• Source biologique: human lymph (axillary)
• Conditionnement: tube of 5 μg 98070109-DNA-5UG; pkg of vial of cells 98070109-1VL
• Mode de croissance: Suspension
• Caryotype: Hyperdiploid, modal no. 47, chromosome 1 trisomy, 15 monosomy, +14q+
• Morphologie: Lymphoblastoid
• Produits: Not specified
• Récepteurs: sIgM,CD22,BB1,HLA-ABC,GnRH
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human B-type EBNA negative lymphoma

Description de la lignée cellulaire

The cell line BHL-89 was established from a 56 year old female patient with chronic lymphoid leukaemia (CLL). The B-cell type cells were shown to express CD9, CD22, CD71,HLA-ABC, BB1, GnRH and surface IgM. Cloning efficiency in soft agar was found to be between 20-60% and population doubling time was 48 hours. BHL-89 cells are Epstein-Barr nuclear antigen (EBNA) negative. They have been shown to be tumourigenic in immunosuppressed mice, sensitive to dibromodulcitol and resistant to the phorbol ester TPA. The cell line was found to be positive for BCL-2 protein and produces small amounts of interferon. Lymphoblastoid cells and floating aggregates. The cell line has been eradicated from mycoplasma at ECACC.

Application

B lymphoma studies, cancer treatments

Milieu de culture

RPMI1640 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Procédure de repiquage

Maintain cultures between 1-5 x 100,000 cells/ml;5% CO₂; 37°C

Autres remarques

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