CESS

92110206, human blood, Lymphoblast

• Code UNSPSC : 41106514

Propriétés

• product name: CESS, 92110206
• Source biologique: human blood
• Mode de croissance: Suspension
• Caryotype: Not specified
• Morphologie: Lymphoblast
• Produits: Surface Immunoglobulin ( Ig), MHC Class II
• Récepteurs: Fc, complement
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human lymphoblast

Description de la lignée cellulaire

Derived from a patient with myelomonocytic leukaemia and subsequently exposed to Epstein Barr Virus (EBV). This line is EBV positive and therefore presumably derived from a normal B lymphocyte. This cell line is also positive for surface Ig, Fc receptor, complement receptor, surface Ia and EBNA. Responds to T cell replacement factor by increasing the number of IgG secreting cells. The cells should be handled under laboratory containment level 2.

Application

T cell replacement factor (TRF) assays, B cell differentiation studies

Profil d'ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 12
D16S539: 12
D5S818: 11,12
D7S820: 10,12
THO1: 7,9.3
TPOX: 8,9
vWA: 16,17

Milieu de culture

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Maintain cultures between 2-9x100,000 cells/ml; 5% CO₂; 37°C.

Autres remarques

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