LL24, 90073001, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
Source biologique
human lung
Conditionnement
tube of 5 μg 90073001-DNA-5UG pkg of vial of cells 90073001-1VL
Derived from normal tissue taken at post-mortem from the lung of a 5 year old male. An ampoule of cells frozen after one passage and estimated population doubling (PDL) 2 was submitted to the American Type Culture Collection in June, 1977. Derived populations at passage 6 and approximately 14 PDL are available for distribution. Progeny were found to undergo an additional 25 population doublings in longevity trials.
Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Finite lifespan, a further 20 population doublings expected.
Autres remarques
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