HTK-

88022409, human bone, Not specified

• Code UNSPSC : 41106514

Propriétés

• product name: HTK-, 88022409
• Source biologique: human bone
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Not specified
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human osteomyeloma

Description de la lignée cellulaire

The cells are rendered TK- by selection with 50μg/ml BUdR. HTK- has the same STR profile as 143B (Sigma Catalogue number 91112502), both are TK-; it is likely that they are the same cell line by different names.

Application

Tumourigenicity studies

Profil d'ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 11
vWA: 18

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 25mg/l Bromo-deoxy Uridine (BUdR) + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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