BC3H1

86093001, mouse brain, Fibroblast

• Code UNSPSC : 41106514

Propriétés

• product name: BC3H1, 86093001
• Source biologique: mouse brain
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Fibroblast
• Produits: Acetylcholine
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse brain tumour

Description de la lignée cellulaire

Established in 1974 from a mouse brain neoplasm tumour induced by nitrosoethylurea, possess many properties characteristic of smooth muscle. Since then it has been suggested that BC3H1 is a skeletal line of ectodermal origin that is defective for commitment to terminal differentiation. The two distinct morphologies typical of this cell line are large spheroid cells and smooth-muscle-like cells. The fusiform is the more common.

Application

Acetylcholine receptor studies.

Milieu de culture

DMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Procédure de repiquage

Spilt confluent cultures 1:3 to 1:10 i.e. seeding at 1-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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